Ultrastructural and cytochemical analysis of physic nut callus tissue in response to different combinations of growth regulators
| dc.creator | Dalilhia Nazaré dos Santos | |
| dc.creator | Claudinéia Ferreira Nunes | |
| dc.creator | Joyce Dória Rodrigues Soares | |
| dc.creator | Eduardo Alves | |
| dc.creator | Cláudia Regina Gontijo Labory | |
| dc.creator | Moacir Pasqual | |
| dc.creator | Leila Aparecida Salles Pio | |
| dc.date | 2015 | |
| dc.date.accessioned | 2026-07-07T03:53:20Z | |
| dc.description | This study aimed to induce callus formation in Jatropha curcas L. and to evaluate the ultrastructure and cytochemical beha vior of the calli. Calluses were induced with 2,4-D, picloram-PIC, kinetin (Kin) and BAP: (1) control; (2) 4.52 μ M 2,4-D; (3) 9.04 μ M 2,4-D; (4) 4.14 μ M PIC; (5) 8.28 μ M PIC; (6) 4.52 μ M 2,4-D + 2.32 μ M KIN; (7) 9.04 μ M 2,4-D + 4.64 μ M KIN; (8) 4.14 μ M PIC + 2.32 μ M KIN; (9) 8.28 μ M PIC + 4.64 μ M KIN; (10) 4.52 μ M 2,4-D + 2.22 μ M BAP; (11) 9.04 μ M 2,4-D + 4.44 μ M BAP; (12) 4.14 μ M PIC + 2.22 μ M BAP and (13) 8.28 μ M PIC + 4.44 μ M BAP. It was evaluated the percent coverage of the explants by callus (% CEC) and performed sca nning electron microscopy (SEM) and acetocarmine/Evans blue double staining to analyze the embryogenic potential of the calli. As shown by scanning electron microscopy and acet ocarmine/Evans blue staining, we found that J. curcas callus formation was optimal with 4.52 μ M of 2,4-D. | |
| dc.format | application/pdf | |
| dc.identifier | 1679-9275 | |
| dc.identifier | https://www.redalyc.org/articulo.oa?id=303041016011 | |
| dc.identifier.uri | http://hdl.handle.net/123456789/446237 | |
| dc.language | en | |
| dc.publisher | Universidade Estadual de Maringá | |
| dc.relation | http://www.redalyc.org/revista.oa?id=3030 | |
| dc.rights | Acta Scientiarum. Agronomy | |
| dc.source | Acta Scientiarum. Agronomy (Brasil) Num.3 Vol.37 | |
| dc.subject | Agrociencias | |
| dc.title | Ultrastructural and cytochemical analysis of physic nut callus tissue in response to different combinations of growth regulators | |
| dc.type | artículo científico |