Ultrastructural and cytochemical analysis of physic nut callus tissue 
in response to different combinations of growth regulators

Dalilhia  Nazaré dos Santos; Claudinéia  Ferreira Nunes; Joyce Dória  Rodrigues Soares; Eduardo  Alves; Cláudia Regina  Gontijo Labory; Moacir  Pasqual; Leila Aparecida  Salles Pio

Ultrastructural and cytochemical analysis of physic nut callus tissue in response to different combinations of growth regulators

Autores

Dalilhia Nazaré dos Santos

Claudinéia Ferreira Nunes

Joyce Dória Rodrigues Soares

Eduardo Alves

Cláudia Regina Gontijo Labory

Moacir Pasqual

Leila Aparecida Salles Pio

Editor

Universidade Estadual de Maringá

Descripción

This study aimed to induce callus formation in Jatropha curcas L. and to evaluate the ultrastructure and cytochemical beha vior of the calli. Calluses were induced with 2,4-D, picloram-PIC, kinetin (Kin) and BAP: (1) control; (2) 4.52 μ M 2,4-D; (3) 9.04 μ M 2,4-D; (4) 4.14 μ M PIC; (5) 8.28 μ M PIC; (6) 4.52 μ M 2,4-D + 2.32 μ M KIN; (7) 9.04 μ M 2,4-D + 4.64 μ M KIN; (8) 4.14 μ M PIC + 2.32 μ M KIN; (9) 8.28 μ M PIC + 4.64 μ M KIN; (10) 4.52 μ M 2,4-D + 2.22 μ M BAP; (11) 9.04 μ M 2,4-D + 4.44 μ M BAP; (12) 4.14 μ M PIC + 2.22 μ M BAP and (13) 8.28 μ M PIC + 4.44 μ M BAP. It was evaluated the percent coverage of the explants by callus (% CEC) and performed sca nning electron microscopy (SEM) and acetocarmine/Evans blue double staining to analyze the embryogenic potential of the calli. As shown by scanning electron microscopy and acet ocarmine/Evans blue staining, we found that J. curcas callus formation was optimal with 4.52 μ M of 2,4-D.