Direct somatic embryogenesis in coffea arabica L. CVS. caturra and catuaí: effect of triacontanol, light condition, and medium consistency
| dc.creator | Andrés M. Gatica | |
| dc.creator | Griselda Arrieta | |
| dc.creator | Ana M. Espinoza | |
| dc.date | 2008 | |
| dc.date.accessioned | 2026-07-07T02:47:17Z | |
| dc.description | The influence of triacontanol (TRIA)concentration and its interaction with indole-3-acetic acid (IAA) on direct somatic embryogenesis(DSE) of Coffea arabica L. cvs Caturra andCatuaí was determined. Additionally, lightconditions and culture medium consistency(semisolid vs. liquid) were evaluated. A higheraverage of somatic embryos per explant fromCaturra (3.9±0.5) and Catuaí (3.6±0.5) leaveswas obtained when 4.55 µM TRIA was addedto the half-strength Murashige and Skoog(1962) medium supplemented with 1.1 µMbenzilaminopurine (BAP) and 2.85 µM IAA.In relation to medium consistency, the highestnumber of embryoids in Caturra (3.2±0.2)and Catuaí (6.0±0.4) explants was obtainedusing Yasuda et al. (1985) semisolid medium.Regarding photoperiod, the number of embryoidsper explant obtained for Catuaí cultured under a16 h light photoperiod was 7.6±1.0 and in thedark 6.2±0.6. In Caturra, 4.2±0.4 embryoidswere produced in the dark and 3.8±0.5 with 16h light. No somatic embryos were observed inCaturra and Catuaí explants after 12 weeks ofculture in liquid Yasuda et al. (1985) medium,and under any of the light conditions. | |
| dc.format | application/pdf | |
| dc.identifier | 0377-9424 | |
| dc.identifier | https://www.redalyc.org/articulo.oa?id=43632113 | |
| dc.identifier.uri | http://hdl.handle.net/123456789/422926 | |
| dc.language | en | |
| dc.publisher | Universidad de Costa Rica | |
| dc.relation | http://www.redalyc.org/revista.oa?id=436 | |
| dc.rights | Agronomía Costarricense | |
| dc.source | Agronomía Costarricense (Costa Rica) Num.1 Vol.32 | |
| dc.subject | Agrociencias | |
| dc.title | Direct somatic embryogenesis in coffea arabica L. CVS. caturra and catuaí: effect of triacontanol, light condition, and medium consistency | |
| dc.type | artículo científico |