Direct somatic embryogenesis in coffea arabica L. CVS. caturra 
and catuaí: effect of triacontanol, light condition, and medium consistency

Andrés M.  Gatica; Griselda  Arrieta; Ana M.  Espinoza

Direct somatic embryogenesis in coffea arabica L. CVS. caturra and catuaí: effect of triacontanol, light condition, and medium consistency

Autores

Andrés M. Gatica

Griselda Arrieta

Ana M. Espinoza

Editor

Universidad de Costa Rica

Descripción

The influence of triacontanol (TRIA)concentration and its interaction with indole-3-acetic acid (IAA) on direct somatic embryogenesis(DSE) of Coffea arabica L. cvs Caturra andCatuaí was determined. Additionally, lightconditions and culture medium consistency(semisolid vs. liquid) were evaluated. A higheraverage of somatic embryos per explant fromCaturra (3.9±0.5) and Catuaí (3.6±0.5) leaveswas obtained when 4.55 µM TRIA was addedto the half-strength Murashige and Skoog(1962) medium supplemented with 1.1 µMbenzilaminopurine (BAP) and 2.85 µM IAA.In relation to medium consistency, the highestnumber of embryoids in Caturra (3.2±0.2)and Catuaí (6.0±0.4) explants was obtainedusing Yasuda et al. (1985) semisolid medium.Regarding photoperiod, the number of embryoidsper explant obtained for Catuaí cultured under a16 h light photoperiod was 7.6±1.0 and in thedark 6.2±0.6. In Caturra, 4.2±0.4 embryoidswere produced in the dark and 3.8±0.5 with 16h light. No somatic embryos were observed inCaturra and Catuaí explants after 12 weeks ofculture in liquid Yasuda et al. (1985) medium,and under any of the light conditions.