Comparison of DNA extraction methods for detection of citrus huanglongbing in Colombia

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dc.creatorJorge Evelio Ángel
dc.creatorErick Geovanni Hernández
dc.creatorNéstor Andrés Herrera
dc.creatorLinda Yhiset Gómez
dc.creatorÁngela Patricia Castro
dc.creatorAdriana Milena Sepúlveda
dc.creatorEverth Emilio Ebratt
dc.date2014
dc.date.accessioned2026-07-07T02:43:15Z
dc.descriptionFour DNA citrus plant tissue extraction protocols and three methods of DNA extraction from vector psyllid Diaphorina citri Kuwayama (Hemiptera: Psyllidae) were compared as part of the validation process and standardization for detection of huanglongbing (HLB). The comparison was done using several criterias such as integrity, purity and concentration. The best quality parameters presented in terms of extraction of DNA from plant midribs tissue of citrus, were cited by Murray and Thompson (1980) and Rodríguez et al. (2010), while for the DNA extraction from psyllid vectors of HLB, the best extraction method was suggested by Manjunath et al. (2008).
dc.formatapplication/pdf
dc.identifier0120-9965
dc.identifierhttps://www.redalyc.org/articulo.oa?id=180330697002
dc.identifier.urihttp://hdl.handle.net/123456789/421798
dc.languageen
dc.publisherUniversidad Nacional de Colombia
dc.relationhttp://www.redalyc.org/revista.oa?id=1803
dc.rightsAgronomía Colombiana
dc.sourceAgronomía Colombiana (Colombia) Num.1 Vol.32
dc.subjectAgrociencias
dc.titleComparison of DNA extraction methods for detection of citrus huanglongbing in Colombia
dc.typeartículo científico

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Agronomía Colombiana - Universidad Nacional de Colombia