Comparison of DNA extraction methods for detection of citrus huanglongbing in Colombia

Jorge Evelio  Ángel; Erick Geovanni  Hernández; Néstor Andrés  Herrera; Linda Yhiset  Gómez; Ángela Patricia  Castro; Adriana Milena  Sepúlveda; Everth Emilio  Ebratt

Comparison of DNA extraction methods for detection of citrus huanglongbing in Colombia

Autores

Jorge Evelio Ángel

Erick Geovanni Hernández

Néstor Andrés Herrera

Linda Yhiset Gómez

Ángela Patricia Castro

Adriana Milena Sepúlveda

Everth Emilio Ebratt

Editor

Universidad Nacional de Colombia

Descripción

Four DNA citrus plant tissue extraction protocols and three methods of DNA extraction from vector psyllid Diaphorina citri Kuwayama (Hemiptera: Psyllidae) were compared as part of the validation process and standardization for detection of huanglongbing (HLB). The comparison was done using several criterias such as integrity, purity and concentration. The best quality parameters presented in terms of extraction of DNA from plant midribs tissue of citrus, were cited by Murray and Thompson (1980) and Rodríguez et al. (2010), while for the DNA extraction from psyllid vectors of HLB, the best extraction method was suggested by Manjunath et al. (2008).