Optimization of invertase assay conditions in rubber tree plants (Hevea brasiliensis Muell. Arg.)

dc.creator	Daria Pimenta de Oliveira
dc.creator	Nelson Delú Filho
dc.creator	Luiz Edson Mota de Oliveira
dc.date	2006
dc.date.accessioned	2026-07-07T03:16:40Z
dc.description	The objective of this work was to define the optimal conditions for invertase assay, seekingto determine the ideal parameters for the different isoenzymes of leaf and bark tissues in adult rubber trees.Assays of varying pH, sucrose concentration and temperature of the reaction medium were conducted forthe two investigated isoenzymes. The results pointed out the existence of two different pH related isoformsfor the two analyzed tissues, with an isoenzyme being more active at pH 5,5 and the other at neutral/alkalinepH. Leaf blade isoenzymes presented similar values for substrate concentration, whereas the bark isoenzymepresented maximum values below those previously reported. The assays at different temperatures presentedsimilar values for leaf isoenzymes, though they have differed significantly among the obtained values.
dc.format	application/pdf
dc.identifier	0100-6762
dc.identifier	https://www.redalyc.org/articulo.oa?id=48853001
dc.identifier.uri	http://hdl.handle.net/123456789/428909
dc.language	en
dc.publisher	Universidade Federal de Viçosa
dc.relation	http://www.redalyc.org/revista.oa?id=488
dc.rights	Revista Árvore
dc.source	Revista Árvore (Brasil) Num.5 Vol.30
dc.subject	Agrociencias
dc.title	Optimization of invertase assay conditions in rubber tree plants (Hevea brasiliensis Muell. Arg.)
dc.type	artículo científico

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