Optimization of invertase assay conditions in rubber tree plants (Hevea brasiliensis Muell. Arg.)
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Universidade Federal de Viçosa
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The objective of this work was to define the optimal conditions for invertase assay, seekingto determine the ideal parameters for the different isoenzymes of leaf and bark tissues in adult rubber trees.Assays of varying pH, sucrose concentration and temperature of the reaction medium were conducted forthe two investigated isoenzymes. The results pointed out the existence of two different pH related isoformsfor the two analyzed tissues, with an isoenzyme being more active at pH 5,5 and the other at neutral/alkalinepH. Leaf blade isoenzymes presented similar values for substrate concentration, whereas the bark isoenzymepresented maximum values below those previously reported. The assays at different temperatures presentedsimilar values for leaf isoenzymes, though they have differed significantly among the obtained values.
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