Comportamento de culturas de bifidobactérias na presença de hidrolisado proteico de soro de leite.
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The behavior of Bifidobacterium bifidum CCT7603, Bifidobacterium animalis subsp. lactis BB12 and Bifidobacterium logum BLG301 in the presence of whey protein hydrolysate (WPH) was evaluated in two steps. Initially, two MRS broth concentrations (30.25 mg/mL and 60.50 mg/mL) were tested with WPH from 0.001 mg/mL to 1 mg/mL. Subsequently, the MRS broth (30.25 mg/mL) was tested with WPH from 0.01 mg/mL to 10 mg/mL. In the first stage, a trend of growth promotion with increasing WPH concentration was only observed for CCT7603 (up to 0.1mg WPH/mL) and BLG301 (WPH to 0.01 mg/mL) cultures. In the second step, two-fold higher absorbance values were obtained for CCT7603 culture in the presence of WPH (up to 1.0 mg/mL) compared to the control. The WPH concentration of 10 mg/mL was inhibitory. Considering the two steps, it seems that the WPH up to 1 mg/mL promoted only the growth of CCT7603 culture.
Palabras clave
Culturas probióticas, Estímulo da multiplicação, Hidrolisado proteico de soro, Probiotic cultures, Whey protein hydrolysate, Bifidobacterium, growth promotion