ALTERNATIVA DE LA TÉCNICA DE TINCIÓN PARA DETERMINAR LA COLONIZACIÓN MICORRÍZICA

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dc.creatorYakelín Rodríguez Yon
dc.creatorLianne Arias Pérez
dc.creatorAida Medina Carmona
dc.creatorYonaisy Mujica Pérez
dc.creatorLaura R. Medina García
dc.creatorKalyanne Fernández Suárez
dc.creatorAracely Mena Echevarría
dc.date2015
dc.date.accessioned2026-07-07T04:25:57Z
dc.descriptionThe root stainning technique is neccesary in the work with arbuscular mycorrhizal fungi (AMF), which constitute the base of several Biofertilizers, as the EcoMic® product, each time more used on agricultural practices at world level. That`s why the sample number evaluated at Mycorrhiza laboratories from diverse Research, Academic and even some productive Centers, increases every year. Those works require the application of such technique; nevertheless, it has the inconvenient to use highly toxic and carcinogenic reagents. The objective of the present work was to substitute those reagents by others non harmful to human health and environment. This is the case of Trypan blue that was substituted by the washable pen ink Parker QuinK. Moreover, Lactic acid and Glycerin were eliminated from the protocol. Results showed the pertinence of such modifications and revealed the usefulness and high quality of mentioned ink, which successfully stains all fungal structures allowing a clear visualization during long time.
dc.formatapplication/pdf
dc.identifier1819-4087
dc.identifierhttps://www.redalyc.org/articulo.oa?id=193239249003
dc.identifier.urihttp://hdl.handle.net/123456789/461493
dc.languagees
dc.publisherInstituto Nacional de Ciencias Agrícolas
dc.relationhttp://www.redalyc.org/revista.oa?id=1932
dc.rightsCultivos Tropicales
dc.sourceCultivos Tropicales (Cuba) Num.2 Vol.36
dc.subjectAgrociencias
dc.titleALTERNATIVA DE LA TÉCNICA DE TINCIÓN PARA DETERMINAR LA COLONIZACIÓN MICORRÍZICA
dc.typeartículo científico

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Cultivos Tropicales - Instituto Nacional de Ciencias Agrícolas