ALTERNATIVA DE LA TÉCNICA DE TINCIÓN PARA DETERMINAR LA COLONIZACIÓN MICORRÍZICA
No hay miniatura disponible
Fecha
Título de la revista
ISSN de la revista
Título del volumen
Editor
Instituto Nacional de Ciencias Agrícolas
Resumen
Descripción
The root stainning technique is neccesary in the work with arbuscular mycorrhizal fungi (AMF), which constitute the base of several Biofertilizers, as the EcoMic® product, each time more used on agricultural practices at world level. That`s why the sample number evaluated at Mycorrhiza laboratories from diverse Research, Academic and even some productive Centers, increases every year. Those works require the application of such technique; nevertheless, it has the inconvenient to use highly toxic and carcinogenic reagents. The objective of the present work was to substitute those reagents by others non harmful to human health and environment. This is the case of Trypan blue that was substituted by the washable pen ink Parker QuinK. Moreover, Lactic acid and Glycerin were eliminated from the protocol. Results showed the pertinence of such modifications and revealed the usefulness and high quality of mentioned ink, which successfully stains all fungal structures allowing a clear visualization during long time.
Palabras clave
Agrociencias