Effects of Dendrobium officinale polysaccharide on adipogenic differentiation of rat bone marrow mesenchymal stem cells
| dc.creator | Yinjuan ZHAO | |
| dc.creator | Lujia XU | |
| dc.creator | Yunfen HUA | |
| dc.date | 2017 | |
| dc.date.accessioned | 2026-07-07T04:22:02Z | |
| dc.description | This study investigated the effect of Dendrobium officinale polysaccharide (D DP) on the adipogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs). D DP was extracted fresh Dendrobium officinale . Rat BMSCs were prepared, and then were treated with 0 (control), 50, 100, 200, 400, 800 μg/mL D DP, respectively. The cell viability was determined by MTT assay. The adipogenic differentiation was quantitatively analyzed by oil red D staining assay. The mRNA expressions of adipogenic differentiation related gene peroxisome proliferator-activated receptor gamma (PPARG), lipoprotein lipase (LPL) and fatty acid binding protein 4 (FABP4) were detected by RT-PCR. Results showed that, D DP with 0-800 μg/mL concentration had no significant toxicity to BMSCs. 200-800 μg/mL D DP could obviously inhibit the adipogenic differentiation of BMSCs. Compared with control group, the expression levels of PPARG, LPL and FABP4 mRNA 200, 400 and 800 μg/mL D DP groups were significantly decreased (P < 0.05 or P < 0.01). D DP can inhibit the adipogenic differentiation of BMSCs, which may be related with its down-regulation of PPARG, LPL and FABP4 expressions in BMSCs. | |
| dc.format | application/pdf | |
| dc.identifier | 0101-2061 | |
| dc.identifier | https://www.redalyc.org/articulo.oa?id=395951059020 | |
| dc.identifier.uri | http://hdl.handle.net/123456789/459359 | |
| dc.language | en | |
| dc.publisher | Sociedade Brasileira de Ciência e Tecnologia de Alimentos | |
| dc.relation | http://www.redalyc.org/revista.oa?id=3959 | |
| dc.rights | Ciência e Tecnologia de Alimentos | |
| dc.source | Ciência e Tecnologia de Alimentos (Brasil) Num.2 Vol.37 | |
| dc.subject | Agrociencias | |
| dc.title | Effects of Dendrobium officinale polysaccharide on adipogenic differentiation of rat bone marrow mesenchymal stem cells | |
| dc.type | artículo científico |