Effects of Dendrobium officinale polysaccharide on adipogenic differentiation of rat bone marrow mesenchymal stem cells
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Sociedade Brasileira de Ciência e Tecnologia de Alimentos
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This study investigated the effect of Dendrobium officinale polysaccharide (D DP) on the adipogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs). D DP was extracted fresh Dendrobium officinale . Rat BMSCs were prepared, and then were treated with 0 (control), 50, 100, 200, 400, 800 μg/mL D DP, respectively. The cell viability was determined by MTT assay. The adipogenic differentiation was quantitatively analyzed by oil red D staining assay. The mRNA expressions of adipogenic differentiation related gene peroxisome proliferator-activated receptor gamma (PPARG), lipoprotein lipase (LPL) and fatty acid binding protein 4 (FABP4) were detected by RT-PCR. Results showed that, D DP with 0-800 μg/mL concentration had no significant toxicity to BMSCs. 200-800 μg/mL D DP could obviously inhibit the adipogenic differentiation of BMSCs. Compared with control group, the expression levels of PPARG, LPL and FABP4 mRNA 200, 400 and 800 μg/mL D DP groups were significantly decreased (P < 0.05 or P < 0.01). D DP can inhibit the adipogenic differentiation of BMSCs, which may be related with its down-regulation of PPARG, LPL and FABP4 expressions in BMSCs.
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