Birth of prolactin receptor-edited Angus calves produced through the electroporation of in vitrofertilized zygotes using CRISPR/Cas9 technology.

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dc.contributorLUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; CAROLINA DAVID VIEIRA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; HYAGO PASSE PEREIRA; DIANA RANGEL DE LEMOS, UNIVERSIDADE FEDERAL DE VIÇOSA; ELIZA DINIZ DE SOUZA; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; NAIARA ZOCCAL SARAIVA, CNPGL; CLARA SLADE OLIVEIRA, CNPGL; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL.
dc.creatorCAMARGO, L. S. de A.
dc.creatorVIEIRA, C. D.
dc.creatorPEREIRA, H. P.
dc.creatorLEMOS, D. R. de
dc.creatorSOUZA, E. D. de
dc.creatorREIS, D. R. de L.
dc.creatorSARAIVA, N. Z.
dc.creatorOLIVEIRA, C. S.
dc.creatorQUINTAO, C. C. R.
dc.creatorSIQUEIRA, L. G. B.
dc.date2025-11-24T18:49:31Z
dc.date2025-11-24T18:49:31Z
dc.date2025-11-24
dc.date2025
dc.date.accessioned2026-07-07T04:00:53Z
dc.descriptionCRISPR/Cas9 technology is a valuable tool for cattle precision breeding. The guide (sgRNA) and Cas9 can be injected into zygote’s cytoplasm; however, it is timing consuming and can impair embryo viability. We have shown that electroporation can be used to deliver sgRNA and Cas9 effectively, promoting gene editing in bovine embryos without affecting their in vitro viability (Camargo et al. 2020 Front Genet 11: 570069). In this study, our objective was to assess whether in vitro-fertilized and electroporated embryos could successfully generate gene-edited Angus calves.
dc.descriptionEdição dos resumos da 38th Annual Meeting of the Brazilian Embryo Technology Society, 2025, Costa do Sauípe, BA.
dc.identifierAnimal Reproduction, v. 22, n. 3, 2025.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1181807
dc.identifier.urihttp://hdl.handle.net/123456789/450552
dc.languageeng
dc.rightsopenAccess
dc.subjectFertilizacao in vitro
dc.subjectEletroporação
dc.subjectBovino
dc.subjectReprodução Animal
dc.subjectEmbrião Animal
dc.subjectMelhoramento Genético Animal
dc.titleBirth of prolactin receptor-edited Angus calves produced through the electroporation of in vitrofertilized zygotes using CRISPR/Cas9 technology.
dc.typeResumo em anais e proceedings

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