Electrophoresis (SDS-PAGE) as a method for screening species of Passiflora using seed proteins as molecular markers.

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dc.contributorMARILIA PENTEADO STEPHAN, CTAA; ANA MARIA COSTA, CPAC; TATIANA DE LIMA AZEVEDO, CTAA; JEANE SANTOS DA ROSA, CTAA; ALEXSANDRO ARAUJO DOS SANTOS, CTAA.
dc.creatorSTEPHAN, M. P.
dc.creatorCOSTA, A. M.
dc.creatorAZEVEDO, T. de L.
dc.creatorROSA, J. S. da
dc.creatorSANTOS, A. A. dos
dc.date2022-09-21T16:05:19Z
dc.date2022-09-21T16:05:19Z
dc.date2022-09-20
dc.date2022
dc.date.accessioned2026-06-30T23:10:27Z
dc.descriptionBrazil is the center of diversity and dispersion of species of the genus Passiflora. Two species of passion fruit Passiflora tenuifila BRS VT (passiflora garlic) and another of Passiflora setacea BRS PC were used in this study. The plants were grown on the Embrapa Cerrados experimental farm. The fruits were washed, minimally cut and their constituents separated. Both species differed in the proportion of wet mass between husk, seed, and pulp. As expected, both species had a higher proportion of husks and seeds and a lower amount of pulp. A semi-purification procedure for the proteins present in the seeds and husks was described and the molecular aspects were analyzed by polyacrylamide gel electrophoresis (SDS-PAGE). The quantification of soluble proteins was performed using the Bradford method. The analysis of the soluble protein extracted from the seeds and husks showed that these values are up to 70 times higher for the seeds of P. tenuifila and 28.5 times for P. setacea, in the husks. It was verified that both species present a similar protein profile, observed by the intense and diverse bands found in the polyacrylamide gel, mainly in the range of 32 to 19 kDa. This work opens up an unexplored field of tracking bioactive proteins and/or peptides including forms of nanostructure systems that protect other bioactive molecules. The objective of this work was to present an analytical procedure to semi-purify seed proteins of two Passiflora species and use electrophoresis as an analysis tool for further screening of their protein profiles and selection of a molecular marker to differentiate them.
dc.identifierJournal of Agricultural Science and Technology B, v. 12, n. 2, p. 40-47, 2022.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1146660
dc.identifierhttps://doi.org/10.17265/2161-6264/2022.02.002
dc.identifier.urihttp://hdl.handle.net/123456789/375774
dc.languageeng
dc.rightsopenAccess
dc.subjectBioactive peptides
dc.subjectP setacea
dc.subjectP tenuifila
dc.subjectMass distribution on fruit
dc.subjectTecnologia de Alimento
dc.subjectMétodo de Análise
dc.subjectEletroforese
dc.subjectPurificação
dc.subjectProteína
dc.subjectPeptídeo
dc.subjectFruto
dc.subjectMaracujá
dc.subjectSemente
dc.subjectCasca
dc.subjectPolpa
dc.subjectRastreamento
dc.subjectFood technology
dc.subjectAnalytical methods
dc.subjectPolyacrylamide gel electrophoresis
dc.subjectPurification methods
dc.subjectProteins
dc.subjectPeptides
dc.subjectFruits
dc.subjectPassion fruits
dc.subjectPulp
dc.subjectPassiflora
dc.subjectSeeds
dc.subjectBioactive compounds
dc.titleElectrophoresis (SDS-PAGE) as a method for screening species of Passiflora using seed proteins as molecular markers.
dc.typeArtigo de periódico

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