Microsatellite markers in maize: challenges and guidelines for implementing multiplex SSR analyses.

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dc.contributorALESSANDRA KOLTUN, UNIVERSIDADE ESTADUAL DE CAMPINAS; PRISCILA ALVES DA SILVA, UNIVERSIDADE ESTADUAL DE CAMPINAS; ISABELA YAMAUCHI TORRES, SEMPRE AGTECH; FERNANDO BONIFÁCIO-ANACLETO, UNIVERSIDADE DE SÃO PAULO; JULIANA ERIKA DE C T YASSITEPE, CNPTIA, UNIVERSIDADE ESTADUAL DE CAMPINAS.
dc.creatorKOLTUN, A.
dc.creatorSILVA, P. A. da
dc.creatorTORRES, I. Y.
dc.creatorBONIFÁCIO-ANACLETO, F.
dc.creatorYASSITEPE, J. E. de C. T.
dc.date2024-04-05T13:35:20Z
dc.date2024-04-05T13:35:20Z
dc.date2024-04-05
dc.date2024
dc.date.accessioned2026-07-07T04:17:45Z
dc.descriptionThis study tested two previously constructed simple sequence repeat (SSR) sets containing ten primer pairs each (ten-plex) in 1142 maize genotypes. Here, we describe the challenges faced when implementing this microsatellite-based genotyping protocol in our laboratory and possible ways to overcome them, hopefully aiding other novice research teams in this field.
dc.identifierCrop Breeding and Applied Biotechnology, v. 24, n. 1, e46392411, 2024.
dc.identifier1984-7033
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1163303
dc.identifierhttp://dx.doi.org/10.1590/1984-70332024v24n1a01
dc.identifier.urihttp://hdl.handle.net/123456789/456803
dc.languagepor
dc.rightsopenAccess
dc.subjectMultiplex PCR
dc.subjectPontuação alélica manual
dc.subjectInterferência de fluoróforo
dc.subjectMilho tropical
dc.subjectMarcadores microssatélites
dc.subjectReação em cadeia da polimerase
dc.subjectManual allele scoring
dc.subjectPull-up
dc.subjectFluorophore interference
dc.subjectTropical maize
dc.subjectPolymerase chain reaction
dc.titleMicrosatellite markers in maize: challenges and guidelines for implementing multiplex SSR analyses.
dc.typeArtigo de periódico

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