Microsatellite markers in maize: challenges and guidelines for implementing multiplex SSR analyses.
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This study tested two previously constructed simple sequence repeat (SSR) sets containing ten primer pairs each (ten-plex) in 1142 maize genotypes. Here, we describe the challenges faced when implementing this microsatellite-based genotyping protocol in our laboratory and possible ways to overcome them, hopefully aiding other novice research teams in this field.
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Multiplex PCR, Pontuação alélica manual, Interferência de fluoróforo, Milho tropical, Marcadores microssatélites, Reação em cadeia da polimerase, Manual allele scoring, Pull-up, Fluorophore interference, Tropical maize, Polymerase chain reaction