Antitumor Effects of Freeze-Dried Robusta Coffee (Coffea canephora) Extracts on Breast Cancer Cell Lines.

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dc.contributorAYELÉN D. NIGRA, Universidad Nacional de Córdoba; DEBORAH DE ALMEIDA BAUER GUIMARÃES, Universidade Federal do Estado do Rio de Janeiro -UNIRIO; CÉSAR G. PRUCCA, Universidad Nacional de Córdoba; OTNIEL FREITAS SILVA, CTAA; ANDERSON J. TEODORO, Universidade Federal do Estado do Rio de Janeiro - UNIRIO; GERMÁN A. GIL, Universidad Nacional de Córdoba.
dc.creatorNIGRA, A. D.
dc.creatorGUIMAREAES, D. de A. B.
dc.creatorPRUCCA, C. G.
dc.creatorFREITAS-SILVA, O.
dc.creatorTEODORO, A. J.
dc.creatorGIL, G. A.
dc.date2021-06-15T12:01:04Z
dc.date2021-06-15T12:01:04Z
dc.date2021-05-21
dc.date2021
dc.date.accessioned2026-06-30T23:10:24Z
dc.descriptionCoffee consumption is believed to have chemopreventive and chemotherapeutic effects and to contribute to preventing thedevelopment and progression of cancer. However, there is still controversy around these claims. As indicated in our previousworks, diet can influence the risk of breast cancer. Intake of coffee is hypothesized to reduce this risk, but current scientificevidence is not conclusive. This work is aimed at studying the effects of Robusta coffee bean extract on cell viability,proliferation, and apoptosis of different human cancers, especially breast cancer cell lines. To this end, cell viability wasevaluated by Alamar Blue in 2D and 3D models, the cell cycle by PI, apoptosis by annexin V, mitochondrial morphology, andfunctionality by mitoTracker, and colony formation capacity by the clonogenic assay. Green and dark coffee extract significantlyreduced viability in human breast, colorectal, brain, and bone cancer cells. Coffee anticancer activity was clearly evidenced inMDA-MB-231 (ER-) and MCF-7 (ER+) breast cancer cells but not in the normal breast cell line. In addition, coffee extractinduces an increase S phase and a decrease G2/M population in breast cancer cells, affected the mitochondrial morphology, andtriggered apoptosis. MDA-MB-231 breast cancer cells lost their clonogenic capacity after treatment. The antitumor activity wasdemonstrated in both 2D and 3D culture cell models
dc.descriptionResearch Article.
dc.identifierOxidative Medicine and Cellular Longevity, 2021, ID 5572630, p. 1-16.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1131938
dc.identifierhttps://doi.org/10.1155/2021/5572630
dc.identifier.urihttp://hdl.handle.net/123456789/375746
dc.languageeng
dc.rightsopenAccess
dc.subjectCafé Robusta
dc.subjectGrão
dc.subjectExtrato Vegetal
dc.subjectCâncer
dc.subjectProduto de Origem Vegetal
dc.subjectFood technology
dc.titleAntitumor Effects of Freeze-Dried Robusta Coffee (Coffea canephora) Extracts on Breast Cancer Cell Lines.
dc.typeArtigo de periódico

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