Polyphasic characterization of bacillus strains isolated from maize.

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dc.contributorUBIRACI GOMES DE PAULA LANA, CNPMS
dc.contributorMARINA ELEUTÉRIO GÓI DE MORAES RODRIGUES, Centro Universitário de Sete Lagoas - UNIFEMM
dc.contributorCHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMS
dc.contributorSYLVIA MORAIS DE SOUSA TINOCO, CNPMS
dc.contributorAMANDA NAYÊ GUIMARÃES TAVARES
dc.contributorIVANILDO EVODIO MARRIEL, CNPMS
dc.contributorELIANE APARECIDA GOMES, CNPMS.
dc.creatorLANA, U. G. de P.
dc.creatorRODRIGUES, M. E. G. de M.
dc.creatorOLIVEIRA-PAIVA, C. A.
dc.creatorSOUSA, S. M. de
dc.creatorTAVARES, A. N. G.
dc.creatorMARRIEL, I. E.
dc.creatorGOMES, E. A.
dc.date2020-11-27T09:01:12Z
dc.date2020-11-27T09:01:12Z
dc.date2020-11-22
dc.date2020
dc.date.accessioned2026-07-07T05:24:37Z
dc.descriptionThe use of bioinoculants with plant growth-promoting bacteria (PGPB) is a sustainable agricultural practice that has been expanding worldwide. Thus, it is fundamental to characterize these bacteria molecularly to provide better security and traceability for bioinoculants production. This work aimed to identify, characterize and develop specific molecular markers for Bacillus strains related to plant-growing promotion. Five strains were identified as B. pumillus (B32), B. thuringiensis (B116), B. megaterium (B119) and B. subtilis (B2082 and B2084), using 16S rRNA gene sequencing, MALDI-TOF mass spectrometry and/or protein crystal detection. Repetitive element palindromic (Rep-PCR) and amplified ribosomal DNA restriction analysis (ARDRA) techniques allowed the discrimination of the strains, except B2082 and B2084 that presented identical genetic profiles, indicating that they are the same or genetically close isolates. Moreover, specific molecular markers were developed for B116 and B119 strains and may be used for microbial inoculants quality control, strains traceability and to detail PGPB colonization.
dc.identifierRevista Brasileira de Milho e Sorgo, v. 19, e1190, 2020.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1126911
dc.identifierhttps://doi.org/10.18512/rbms2020v19e1190
dc.identifier.urihttp://hdl.handle.net/123456789/490209
dc.languageeng
dc.rightsopenAccess
dc.subjectPerfil molecular
dc.subjectCrescimento de plantas
dc.subjectBactéria
dc.subjectZea Mays
dc.titlePolyphasic characterization of bacillus strains isolated from maize.
dc.typeArtigo de periódico

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