Polyphasic characterization of bacillus strains isolated from maize.

LANA, U. G. de P.; RODRIGUES, M. E. G. de M.; OLIVEIRA-PAIVA, C. A.; SOUSA, S. M. de; TAVARES, A. N. G.; MARRIEL, I. E.; GOMES, E. A.

Polyphasic characterization of bacillus strains isolated from maize.

Autores

LANA, U. G. de P.

RODRIGUES, M. E. G. de M.

OLIVEIRA-PAIVA, C. A.

Descripción

The use of bioinoculants with plant growth-promoting bacteria (PGPB) is a sustainable agricultural practice that has been expanding worldwide. Thus, it is fundamental to characterize these bacteria molecularly to provide better security and traceability for bioinoculants production. This work aimed to identify, characterize and develop specific molecular markers for Bacillus strains related to plant-growing promotion. Five strains were identified as B. pumillus (B32), B. thuringiensis (B116), B. megaterium (B119) and B. subtilis (B2082 and B2084), using 16S rRNA gene sequencing, MALDI-TOF mass spectrometry and/or protein crystal detection. Repetitive element palindromic (Rep-PCR) and amplified ribosomal DNA restriction analysis (ARDRA) techniques allowed the discrimination of the strains, except B2082 and B2084 that presented identical genetic profiles, indicating that they are the same or genetically close isolates. Moreover, specific molecular markers were developed for B116 and B119 strains and may be used for microbial inoculants quality control, strains traceability and to detail PGPB colonization.