Genome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja Juz. et Buk.

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dc.creatorDaniela Vallejo C.
dc.creatorPablo Andrés Gutiérrez S.
dc.creatorMauricio Marín M.
dc.date2016
dc.date.accessioned2026-07-07T02:43:43Z
dc.descriptionPotato virus S (PVS) is a prevalent virus in potato fields in Colombia and the rest of the world. PVS has been classified into two separate lineages, PVS O (Ordinary) and PVS A (An - dean), which are genetically distinct. In this study, the com - plete genome sequence of a new PVS isolate (PVS_Antioquia) was obtained using High-throughput sequencing (Illumina HiSeq-2000) from tuber sprouts of Solanum phureja (var. Criolla Colombia). The PVS_Antioquia genome comprises 8,483 nt that code for six ORFs: RdRp (223 kDa), TGBp1-3 (25kDa, 12kDa, 7kDa) CP (32.3 kDa) and NABP (11 kDa) and share a high sequence identity with respect to the PVS_RVC (>95%) from Colombia, in contrast to 81 to 82% identity with respect to the PVS A and PVS O isolates from around the world. This genome information was used to design RT-qPCR prim - ers that are specific for the Colombian PVS strains (RVC and Antioquia) which were validated in S. phureja leaf and tuber samples. These primers detected PVS in 80 and 60% of a set of fifteen leaf and tuber samples, respectively, suggesting a high incidence of this virus in the potato crops of Antioquia.
dc.formatapplication/pdf
dc.identifier0120-9965
dc.identifierhttps://www.redalyc.org/articulo.oa?id=180345625007
dc.identifierhttps://www.redalyc.org/journal/1803/180345625007/
dc.identifierhttps://www.redalyc.org/journal/1803/180345625007/html/
dc.identifierhttps://www.redalyc.org/journal/1803/180345625007/180345625007.epub
dc.identifierhttps://www.redalyc.org/journal/1803/180345625007/movil
dc.identifier.urihttp://hdl.handle.net/123456789/422031
dc.languageen
dc.publisherUniversidad Nacional de Colombia
dc.relationhttp://www.redalyc.org/revista.oa?id=1803
dc.rightsAgronomía Colombiana
dc.sourceAgronomía Colombiana (Colombia) Num.1 Vol.34
dc.subjectAgrociencias
dc.titleGenome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja Juz. et Buk.
dc.typeartículo científico

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Agronomía Colombiana - Universidad Nacional de Colombia