Genome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja Juz. et Buk.
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Universidad Nacional de Colombia
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Potato virus S (PVS) is a prevalent virus in potato fields in Colombia and the rest of the world. PVS has been classified into two separate lineages, PVS O (Ordinary) and PVS A (An - dean), which are genetically distinct. In this study, the com - plete genome sequence of a new PVS isolate (PVS_Antioquia) was obtained using High-throughput sequencing (Illumina HiSeq-2000) from tuber sprouts of Solanum phureja (var. Criolla Colombia). The PVS_Antioquia genome comprises 8,483 nt that code for six ORFs: RdRp (223 kDa), TGBp1-3 (25kDa, 12kDa, 7kDa) CP (32.3 kDa) and NABP (11 kDa) and share a high sequence identity with respect to the PVS_RVC (>95%) from Colombia, in contrast to 81 to 82% identity with respect to the PVS A and PVS O isolates from around the world. This genome information was used to design RT-qPCR prim - ers that are specific for the Colombian PVS strains (RVC and Antioquia) which were validated in S. phureja leaf and tuber samples. These primers detected PVS in 80 and 60% of a set of fifteen leaf and tuber samples, respectively, suggesting a high incidence of this virus in the potato crops of Antioquia.
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