OPTIMIZATION OF RANDOM AMPLIFIED POLYMORPHIC DNA PROTOCOL FOR MOLECULAR IDENTIFICATION OF Lophius gastrophysus

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dc.creatorMicheline S. RAMELLA
dc.creatorMariela A. KROTH
dc.creatorCaroline TAGLIARI
dc.creatorAna Carolina M. ARISI
dc.date2005
dc.date.accessioned2026-07-07T04:17:17Z
dc.descriptionIn this study, we described the optimization of Random Amplified Polymorphic DNA (RAPD) protocol for identification of L. gastrophysus. Different conditions (annealing temperatures, MgCl2 concentrations, DNA quantity) were tested to find reproducible and adequate profiles. Amplifications performed with primers A01, A02 and A03 generate the best RAPD profiles when the conditions were annealing temperature of 36ºC, 25 ng of DNA quantity and 2.5 mM MgCl2. Exact identification of the species and origin of marine products is necessary and RAPD could be used as an accurate, rapid tool to expose commercial fraud.
dc.formatapplication/pdf
dc.identifier0101-2061
dc.identifierhttps://www.redalyc.org/articulo.oa?id=395940076017
dc.identifier.urihttp://hdl.handle.net/123456789/456484
dc.languageen
dc.publisherSociedade Brasileira de Ciência e Tecnologia de Alimentos
dc.relationhttp://www.redalyc.org/revista.oa?id=3959
dc.rightsCiência e Tecnologia de Alimentos
dc.sourceCiência e Tecnologia de Alimentos (Brasil) Num.4 Vol.25
dc.subjectAgrociencias
dc.titleOPTIMIZATION OF RANDOM AMPLIFIED POLYMORPHIC DNA PROTOCOL FOR MOLECULAR IDENTIFICATION OF Lophius gastrophysus
dc.typeartículo científico

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Ciência e Tecnologia de Alimentos - Sociedade Brasileira de Ciência e Tecnologia de Alimentos