OPTIMIZATION OF RANDOM AMPLIFIED POLYMORPHIC DNA PROTOCOL FOR MOLECULAR IDENTIFICATION OF Lophius gastrophysus
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Sociedade Brasileira de Ciência e Tecnologia de Alimentos
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In this study, we described the optimization of Random Amplified Polymorphic DNA (RAPD) protocol for identification of L. gastrophysus. Different conditions (annealing temperatures, MgCl2 concentrations, DNA quantity) were tested to find reproducible and adequate profiles. Amplifications performed with primers A01, A02 and A03 generate the best RAPD profiles when the conditions were annealing temperature of 36ºC, 25 ng of DNA quantity and 2.5 mM MgCl2. Exact identification of the species and origin of marine products is necessary and RAPD could be used as an accurate, rapid tool to expose commercial fraud.
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