In vitro tuberization in white yam (Dioscorea rotundata Poir)
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Springer
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Nodal cuttings of white yam were induced to produce microtubers on a MS-revised medium supplemented with various concentrations of sucrose, 20 mgl−1 L-cysteine, 0.5 mgl−1 kinetin and 0.7% agar. The frequency of tuberization was affected by the daylength, which is optimal at 12 and 16 h of light depending on the sucrose concentration. The microtubers were planted in a seed bed and grown to maturity. The importance of in vitro tuberization of yam as a means of international germplasm distribution or exchange as well as for the propagation of planting material is discussed.
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dioscorea rotundata, yams, sucrose, germplasm exchange, propagation, in vitro, tubers