Elevation of condensed tannins in the leaves of Ta-MYB14-1 white clover (Trifolium repens L.) outcrossed with high anthocyanin lines

Vista sencilla de ítem
dc.creatorRoldan, M.
dc.creatorCousins, G.
dc.creatorFraser, K.
dc.creatorHancock, K.
dc.creatorCollette, V.
dc.creatorDemmer, J.
dc.creatorDerek, R.W.
dc.creatorCaradus, J.
dc.creatorJones, Christopher S.
dc.creatorVoisey, C.
dc.date2020-03-11
dc.date2019-07-25T11:40:34Z
dc.date2019-07-25T11:40:34Z
dc.date.accessioned2026-06-27T17:12:30Z
dc.descriptionCondensed tannins (CT) are highly desirable in forage as they sequester dietary protein and reduce bloat and methane emissions in ruminants. However, the widely used forage legume white clover (Trifolium repens) only produces CTs in flowers and trichomes and at levels too low to achieve therapeutic effects. Genetic transformation with transcription factor Ta-MYB14-1 from Trifolium arvense was effective in inducing CTs to 0.6% of leaf dry matter. CT synthesis has been elevated further by crossing the primary white clover transgenic line with wild type genotypes producing the related phenylpropanoids, anthocyanins. CT levels in leaves were highest under the anthocyanin leaf marks associated with the “red midrib” trait; however, there was no evidence for CT accumulation in leaf sections with the “red V” anthocyanin marking. Ta-MYB14-1 was stably inherited in two generations of crosses, and T2 progeny produced up to 3.6-fold higher CTs than the T0 parent. The profile of small CT oligomers such as dimers and trimers was consistent in T0, T1, T2, and BC2 progeny and consisted predominantly of prodelphinidins (PD), with lesser amounts of procyanidins (PC) and mixed PC:PD oligomers.
dc.identifierhttps://hdl.handle.net/10568/102317
dc.identifier.urihttp://hdl.handle.net/123456789/145431
dc.languageen
dc.publisherAmerican Chemical Society
dc.rightsOpen Access
dc.sourceRoldan, M., Cousins, G., Fraser, K., Hancock, K., Collette, V., Demmer, J., Derek, R.W., Caradus, J., Jones, C.S. and Voisey, C. 2019. Elevation of condensed tannins in the leaves of Ta-MYB14-1 white clover (Trifolium repens L.) outcrossed with high anthocyanin lines. Journal of Agricultural and Food Chemistry
dc.subjectmixed farming
dc.subjectleaves
dc.subjecttannins
dc.subjecttrifolium repens
dc.subjectanthocyanins
dc.titleElevation of condensed tannins in the leaves of Ta-MYB14-1 white clover (Trifolium repens L.) outcrossed with high anthocyanin lines
dc.typeJournal Article

Archivos

Colecciones

Instituto Internacional de Investigaciones Pecuarias (ILRI)