Treatment of cattle with DNA-encoded Flt3L and GM-CSF prior to immunization with Theileria parva candidate vaccine antigens induces CD4 and CD8 T cell IFN-γ responses but not CTL responses

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dc.creatorMwangi, David Miano
dc.creatorHonda, Y.
dc.creatorGraham, S.P.
dc.creatorPelle, Roger
dc.creatorTaracha, E.L.N.
dc.creatorGachanja, J.
dc.creatorNyanjui, J.K.
dc.creatorBray, J.
dc.creatorPalmer, G.H.
dc.creatorBrown, W.C.
dc.creatorMwangi, W.
dc.date2011-04
dc.date2011-01-21T09:49:52Z
dc.date2011-01-21T09:49:52Z
dc.date.accessioned2026-06-27T16:36:27Z
dc.descriptionTheileria parva antigens recognized by cytotoxic T lymphocytes (CTLs) are prime vaccine candidates against East Coast Fever in cattle. A strategy for enhancing induction of parasite-specific T cell responses by increasing recruitment and activation of dendritic cells (DCs) at the immunization site by administration of bovine Flt3L and GM-CSF prior to inoculation with DNA vaccine constructs and MVA boost was evaluated. Analysis of immune responses showed induction of significant T. parva-specific proliferation, and IFN-γ-secreting CD4+ and CD8+ T cell responses in immunized cattle. However, antigen-specific CTLs were not detected. Following lethal challenge, 5/12 immunized cattle survived by day 21, whereas all the negative controls had to be euthanized due to severe disease, indicating a protective effect of the vaccine (p < 0.05). The study demonstrated the potential of this technology to elicit significant MHC class II and class I restricted IFN-γ-secreting CD4+ and CD8+ T cells to defined vaccine candidate antigens in a natural host, but also underscores the need to improve strategies for eliciting protective CTL responses.
dc.identifierhttps://hdl.handle.net/10568/3074
dc.identifier.urihttp://hdl.handle.net/123456789/127970
dc.languageen
dc.publisherElsevier
dc.rightsLimited Access
dc.sourceMwangi, D.M., Yoshikazu Honda, Graham, S.P., Pelle, R., Taracha, E.L.N., Gachanja, J., Nyanjui, J.K., Bray, J., Palmer, G.H., Brown, W.C. and Mwangi, W. 2011. Treatment of cattle with DNA-encoded Flt3L and GM-CSF prior to immunization with Theileria parva candidate vaccine antigens induces CD4 and CD8 T cell IFN-γ responses but not CTL responses. Veterinary Immunology and Immunopathology 140(3-4): 244-251.
dc.subjectgenetics
dc.subjectanimal diseases
dc.subjectimmunology
dc.titleTreatment of cattle with DNA-encoded Flt3L and GM-CSF prior to immunization with Theileria parva candidate vaccine antigens induces CD4 and CD8 T cell IFN-γ responses but not CTL responses
dc.typeJournal Article

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