High resolution melting analysis on temperature allows the genotype determination of indicators related to beef

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dc.creatorL. E. López Rojas
dc.creatorLaura Patiño Cadavid
dc.creatorJuliana María Martínez Garro
dc.creatorSilvana Durán Ortiz
dc.creatorLina Johana Correa Agudelo
dc.creatorSantiago González Escudero
dc.creatorAlbeiro López Herrera
dc.creatorJosé Julián Echeverri Zuluaga
dc.date2016
dc.date.accessioned2026-07-07T03:35:21Z
dc.descriptionSome SNPs within μ-calpain (CAPN1), calpastatin (CAST) and leptin (LEP) genes have been associated to beef tenderness and, in order to optimize the processes of genetic improvement in cattle rearing farms, it is necessary to apply fast, reliable and accessible methodologies to producers, for determining the genotype of these polymorphisms. Although there are several methodologies, the HRM is stated as a promising alternative that meets these conditions. Therefore, the PCR-HRM was standardized and validated for genotyping the SNPs CAPN4751, CAPN316, CAST2959, CAST282, E2FB and E2JW of 50 animals from Bos taurus and Bos indicus breeds, using PCR-RFLP as gold standard test. There was an agreement among the results obtained by PCRHRM, compared to PCR-RFLP, and high sensitivity, specificity and appropriate negative and positive predictive values for PCRHRM. Similarly, repeatability and reproducibility were verified after obtaining homogeneity in the intra-run and inter-run results. Although the results show that the PCR-HRM is an efficient technique, and allows to obtain reliable results in a short time, these can be affected by the extraction method, quality and quantity of DNA, among other undetermined factors that can generate false positives and atypical dissociation profiles. Therefore, these variables must be controlled and the PCR should be monitored to identify unusual dissociation curves, caused by excess or deficiency in the amplification.
dc.formatapplication/pdf
dc.identifier0034-7485
dc.identifierhttps://www.redalyc.org/articulo.oa?id=193045926003
dc.identifier.urihttp://hdl.handle.net/123456789/438160
dc.languageen
dc.publisherInstituto de Ciencia Animal
dc.relationhttp://www.redalyc.org/revista.oa?id=1930
dc.rightsRevista Cubana de Ciencia Agrícola
dc.sourceRevista Cubana de Ciencia Agrícola (Cuba) Num.1 Vol.50
dc.subjectAgrociencias
dc.titleHigh resolution melting analysis on temperature allows the genotype determination of indicators related to beef
dc.typeartículo científico

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Revista Cubana de Ciencia Agrícola - Instituto de Ciencia Animal