Overexpression of a high-affinity nitrate transporter OsNRT2.1 increases yield and manganese accumulation in rice under alternating wet and dry condition

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dc.creatorLuo, Bingbing
dc.creatorChen, Jingguang
dc.creatorZhu, Longlong
dc.creatorLiu, Shuhua
dc.creatorLi, Bin
dc.creatorLu, Hong
dc.creatorYe, Guoyou
dc.creatorXu, Guohua
dc.creatorFan, Xiaorong
dc.date2018-08-15
dc.date2024-12-19T12:54:20Z
dc.date2024-12-19T12:54:20Z
dc.date.accessioned2026-06-27T04:16:14Z
dc.descriptionNitrate and manganese (Mn) are necessary elements for the growth and development of rice in paddy soil. Under physiological conditions, we previously reported that the uptake of Mn in roots can be improved by the addition of external nitrate but not ammonium. To investigate the mechanism(s) of this phenotype, we produced plant lines overexpressing OsNRT2.1 and assessed Mn uptake under alternating wet and dry (AWD) and waterlogged (WL) conditions. Under AWD condition, we observed a 31% reduction in grain yields of wild type (WT) plants compared to WL condition. Interestingly, the overexpression of OsNRT2.1 could recover this loss, as OsNRT2.1 transgenic lines displayed higher grain yields than WT plants. We also observed 60% higher grain Mn in the transgenic lines in AWD condition and approximately 30% higher Mn in the grain of transgenic lines in WL condition. We further found that the overexpression of OsNRT2.1 did not alter Mg and Fe in the seeds in either growth condition. The reasons for the increased Mn content in OsNRT2.1 transgenic seeds in AWD condition could be explained by the elevated expression of OsNRAMP family genes including OsNRAMP3, OsNRAMP5, and OsNRAMP6 in node I, the panicle-neck, and the flag leaves. The mechanism(s) underpinning the upregulation of these genes requires further investigation. Taken together, our results provide a new function of OsNRT2.1 in improving rice yields and grain Mn accumulation during water-saving cultivation patterns. This represents a new strategy for maintaining yield and improving food quality in a sustainable agricultural system.
dc.identifierhttps://hdl.handle.net/10568/164817
dc.identifier.urihttp://hdl.handle.net/123456789/27213
dc.languageen
dc.publisherFrontiers Media
dc.rightsOpen Access
dc.sourceLuo, Bingbing; Chen, Jingguang; Zhu, Longlong; Liu, Shuhua; Li, Bin; Lu, Hong; Ye, Guoyou; Xu, Guohua and Fan, Xiaorong. 2018. Overexpression of a high-affinity nitrate transporter OsNRT2.1 increases yield and manganese accumulation in rice under alternating wet and dry condition. Front. Plant Sci., Volume 9
dc.subjectalternate wetting and drying
dc.subjectgenetic expression
dc.subjectmanganese
dc.subjectnitrates
dc.subjecttransporters
dc.subjectyields
dc.titleOverexpression of a high-affinity nitrate transporter OsNRT2.1 increases yield and manganese accumulation in rice under alternating wet and dry condition
dc.typeJournal Article

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Instituto Internacional de Investigación del Arroz (IRRI)