Diagnostic kit for rice blight resistance

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dc.creatorEom, Joon-Seob
dc.creatorLuo, Dangping
dc.creatorAtienza-Grande, Genelou
dc.creatorYang, Jungil
dc.creatorJi, Chonghui
dc.creatorThi Luu, Van
dc.creatorHuguet-Tapia, José C.
dc.creatorChar, Si Nian
dc.creatorLiu, Bo
dc.creatorNguyen, Hanna
dc.creatorSchmidt, Sarah Maria
dc.creatorSzurek, Boris
dc.creatorVera Cruz, Casiana
dc.creatorWhite, Frank F.
dc.creatorOliva, Ricardo
dc.creatorYang, Bing
dc.creatorFrommer, Wolf B.
dc.date2019-11
dc.date2024-12-19T12:54:06Z
dc.date2024-12-19T12:54:06Z
dc.date.accessioned2026-06-27T04:12:06Z
dc.descriptionBlight-resistant rice lines are the most effective solution for bacterial blight, caused byXanthomonas oryzaepv.oryzae(Xoo). Key resistance mechanisms involveSWEETgenes as susceptibility factors. Bacterial transcription activator-like (TAL) effectors bind to effector-binding elements (EBEs) inSWEETgene promoters and induceSWEETgenes. EBE variants that cannot be recognized by TAL effectors abrogate induction, causing resistance. Here we describe a diagnostic kit to enable analysis of bacterial blight in the field and identification of suitable resistant lines. Specifically, we include aSWEETpromoter database, RT–PCR primers for detectingSWEETinduction, engineered reporter rice lines to visualize SWEET protein accumulation and knock-out rice lines to identify virulence mechanisms in bacterial isolates. We also developed CRISPR–Cas9 genome-edited Kitaake rice to evaluate the efficacy of EBE mutations in resistance, software to predict the optimal resistance gene set for a specific geographic region, and two resistant ‘mega’ rice lines that will empower farmers to plant lines that are most likely to resist rice blight.
dc.identifierhttps://hdl.handle.net/10568/164606
dc.identifier.urihttp://hdl.handle.net/123456789/24903
dc.languageen
dc.publisherSpringer
dc.rightsOpen Access
dc.sourceEom, Joon-Seob; Luo, Dangping; Atienza-Grande, Genelou; Yang, Jungil; Ji, Chonghui; Thi Luu, Van; Huguet-Tapia, José C.; Char, Si Nian; Liu, Bo; Nguyen, Hanna; Schmidt, Sarah Maria; Szurek, Boris; Vera Cruz, Casiana; White, Frank F.; Oliva, Ricardo; Yang, Bing and Frommer, Wolf B. 2019. Diagnostic kit for rice blight resistance. Nat Biotechnol, Volume 37 no. 11 p. 1372-1379
dc.subjectapplied microbiology and biotechnology
dc.subjectbioengineering
dc.subjectbiomedical engineering
dc.subjectbiotechnology
dc.subjectmolecular medicine
dc.titleDiagnostic kit for rice blight resistance
dc.typeJournal Article

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Instituto Internacional de Investigación del Arroz (IRRI)