New EST-SSR markers of Coffea arabica: transferability and application to studies of molecular characterization and genetic mapping.

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dc.contributorLUÍS FELIPE V. FERRÃO, UFV; EVELINE TEIXEIRA CAIXETA, SAPC; GUILHERME PENA, UFV; EUNICE M. ZAMBOLIM, UFV; COMES D. CRUZ, UFV; LAÉRCIO ZAMBOLIM, UFV; MARIA AMELIA GAVA FERRAO, SAPC; NEY S. SAKIYAMA, UFV.
dc.creatorFERRÃO, L. F. V.
dc.creatorCAIXETA, E. T.
dc.creatorPENA, G.
dc.creatorZAMBOLIM, E. M.
dc.creatorCRUZ, C. D.
dc.creatorZAMBOLIM, L.
dc.creatorFERRAO, M. A. G.
dc.creatorSAKIYAMA, N. S.
dc.date2016-03-14T11:11:11Z
dc.date2016-03-14T11:11:11Z
dc.date2016-03-14
dc.date2015
dc.date2016-03-22T11:11:11Z
dc.date.accessioned2026-07-01T00:26:16Z
dc.descriptionMicrosatellite markers (SSR) have broad utility in genetic studies due to a high rate of polymor-phisms, a codominant nature and multiallelism. EST?SSRs are markers derived from the expressed sequences of a genome and represent transcribed genes. Despite the importance of the genus Coffea , only a small number of EST?SSR markers are currently available. Thus, this study was designed to mine and develop a new set of EST?SSR markers from the Brazilian Coffee Genome Project. We investigated 130,792 expressed sequence tags (ESTs), from which 24,031 DNA sequences with microsatellites were identified. After stability and amplification testing, 101 new EST?SSR markers were developed and analyzed in different coffee species. The average rate of transferability was 88 %, showing that these markers are useful in genetic studies across the genus Coffea. Polymorphism levels and the degree of diversity were consistent with the evolutionary history of the species. All coffee genotypes were discriminated, even the C. arabica genotypes that have known narrow genetic basis. It was also possible to locate 14 EST? SSRs into different linkage groups of the C. arabica genetic map, which demonstrate that these markers can be useful in QTL mapping studies and in molecular-assisted selection.
dc.identifierMolecular Breeding, v. 35, n. 1, p. 2-5, jan. 2015.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1040767
dc.identifier.urihttp://hdl.handle.net/123456789/403999
dc.languageeng
dc.rightsopenAccess
dc.subjectBrazilian Coffee Genome Project
dc.subjectMicrosatellites
dc.subjectCoffea Arábica
dc.subjectGenetic markers
dc.subjectCoffea
dc.titleNew EST-SSR markers of Coffea arabica: transferability and application to studies of molecular characterization and genetic mapping.
dc.typeArtigo de periódico

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