Selection of Bacillus thuringiensis strains toxic to                         Meloidogyne incognita

Jônatas Barros dos  Santos; Alberto do Nascimento  Silva; Paulo Roberto Martins  Queiroz; Barbara  Eckstein; Rose Gomes  Monnerat

Selection of Bacillus thuringiensis strains toxic to Meloidogyne incognita

Autores

Jônatas Barros dos Santos

Alberto do Nascimento Silva

Paulo Roberto Martins Queiroz

Barbara Eckstein

Rose Gomes Monnerat

Editor

Universidade Federal de Goiás

Descripción

The Bacillus thuringiensis bacterium has demonstrated an effective potential in the control of several agricultural pests, among them nematodes. This study aimed to standardize and establish a methodology of bioassays of B. thuringiensis and Meloidogyne incognita with the use of resorcinol, select B. thuringiensis strains toxic to M. incognita in vitro and molecularly identify the presence of the cry6 gene in B. thuringiensis strains. Second-stage juveniles were subjected to resorcinol doses, verifying that the concentration of 0.2 % did not cause mortality and provided the Cry6A toxin ingestion. Thereafter, 16 B. thuringiensis strains were tested in the presence or absence of resorcinol, resulting in mortality rates of 4-36 %. Among the B. thuringiensis strains analyzed for the presence of the cry6 gene, only one was detected (S1617).