Population genetics of the sugarcane borer Diatraea saccharalis (Fabr.) (Lepidoptera: Crambidae)

Denise  Alves Lopes; Liriana  Belizário Cantagalli; Ana Lúcia Paz  Barateiro Stuchi; Claudete  Aparecida Mangolin; Maria Claudia Colla  Ruvolo-Takasusuki

Population genetics of the sugarcane borer Diatraea saccharalis (Fabr.) (Lepidoptera: Crambidae)

Autores

Denise Alves Lopes

Liriana Belizário Cantagalli

Ana Lúcia Paz Barateiro Stuchi

Claudete Aparecida Mangolin

Maria Claudia Colla Ruvolo-Takasusuki

Editor

Universidade Estadual de Maringá

Descripción

Diatraea saccharalis is the principal pest of sugarcane in Brazil and is found throughout the sugarcane crop. Information about its population genetics is scarce, but population genetic analysis is of particular importance as a basis for a successful pest control program. Pest control requires a constant evaluation of genetic variability so that appropriate strategies can be employed. In this study, the structure of D. saccharalis populations in sugarcane crops was analyzed with PCR-RAPD (Polymerase Chain Reaction-Random Amplified Polymorphic DNA) markers. Samples were collected from four sugarcane fields in ParanáState and one in São PauloState. Twelve PCR-RAPD primers were used. Th e analysis produced 216 fragments, with a 99.54% polymorphism rate. The Shannon index averaged 0.3797 (± 0.1729), and the average value of G ST for the five populations was 0.0909. These results showed that the populations are differentiated. An analysis of molecular variance showed that 92% of the poly morphism occurs within populations. The high polymorphism rate within these populations shows that these insects have the potentia l to develop resistance to insecticides. The control and management of D. saccharalis should be conducted cautiously and integrated using chemical and biological control.