A novel soybean hairy root system for gene functional validation.

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dc.contributorBRUNA MEDEIROS PEREIRA, UNIVERSIDADE DE BRASÍLIA
dc.contributorFABRÍCIO ARRAES, UNIVERSIDADE DE BRASILIA
dc.contributorANDRESSA CUNHA QUINTANA MARTINS, UNIVERSIDADE CATOLICA DE BRASILIA
dc.contributorNAYARA SABRINA FREITAS ALVES, UNIVERSIDADE FEDERAL DO PARANÁ
dc.contributorBRUNO PAES MELO, LONGPING HIGH TECH
dc.contributorCAROLINA VIANNA MORGANTE, CPATSA
dc.contributorMARIO ALFREDO PASSOS SARAIVA, INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIA
dc.contributorMARIA FATIMA GROSSI DE SA, CENARGEN
dc.contributorPATRICIA MESSEMBERG GUIMARAES, CENARGEN
dc.contributorANA CRISTINA MIRANDA BRASILEIRO, CENARGEN.
dc.creatorPEREIRA, B. M.
dc.creatorARRAES, F.
dc.creatorMARTINS, A. C. Q.
dc.creatorALVES, N. S. F.
dc.creatorMELO, B. P.
dc.creatorMORGANTE, C. V.
dc.creatorSARAIVA, M. A. P.
dc.creatorSA, M. F. G. de
dc.creatorGUIMARAES, P. M.
dc.creatorBRASILEIRO, A. C. M.
dc.date2023-05-29T14:47:59Z
dc.date2023-05-29T14:47:59Z
dc.date2023-05-29
dc.date2023
dc.date.accessioned2026-07-07T05:57:54Z
dc.descriptionAgrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suitable for high throughput in root analysis of candidate genes in soybean.
dc.descriptionNa publicação: Maria Fátima Grossi-de-Sa; Patricia Messenberg Guimaraes.
dc.identifierPlos One, v. 18, n. 5, e0285504, 2023.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1154066
dc.identifierhttps://doi. org/10.1371/journal.pone.0285504
dc.identifier.urihttp://hdl.handle.net/123456789/503494
dc.languageeng
dc.rightsopenAccess
dc.subjectTransgenic soybean
dc.subjectAgrobacterium rhizogenes
dc.subjectGlycine Max
dc.subjectSoja
dc.subjectMelhoramento Genético Vegetal
dc.subjectMeloidogyne spp
dc.subjectSoybeans
dc.titleA novel soybean hairy root system for gene functional validation.
dc.typeArtigo de periódico

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