Non-destructive determination of photosynthetic pigments in the leaves of castor oil plants
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Universidade Estadual de Maringá
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The aim of the present work was to determ ine the relationship between photosynthetic pigments extracted in dimethyl sulfoxide (DMSO) an d readings obtained by the portable chlorophyll meter, ClorofiLOG ® 1030, to generate mathematical models th at are able to report the contents of chlorophyll and carotenoids in the leaves of the cast or oil plant. This work was conducted at Embrapa Cotton, located in the city of Camp ina Grande, state of Paraíba, Brazil, in October 2010. For the indirect analysis, portable equipment was used to read leaf di scs of different shades of green. The chlorophyll in these discs was then determined us ing a classical method: 5 mL of DM SO was added, the samples were incubated in a water bath at 70ºC for 30 minutes, and a 3 mL aliquot was removed for spectrophotometric measurements at 470, 646 and 663 nm. Th e data were subjected to analys is of variance and regression analyses in which the readings obta ined using the portable chlorophyll meter were the dependent variable and the photosynthetic pigments th at were determined by the classi cal method were the independent variable. The results indicated that, with the exception of chlorophyll b , the ClorofiLOG ® 1030 portable chlorophyll meter estimated the concentration of photosyn thetic pigments with high precision, thus saving time and the chemical reagen ts that are typically used in conventional procedures.
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