Host/nonhost status and genetics of resistance in barley against three pathotypes of Magnaporthe blast fungi.
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Blast disease, caused by the Magnapor the oryzae/griseaspecies complex, occurs in a wide rangeof wild and cultivated gramineous plant species including rice, wheat and barley. We inoculated a collection of cultivated (Hordeum vulgaressp.vul-gareL.) and wild (ssp.spontaneum) barley accessions with M. oryzae Oryzapathotype (MoO),Triticumpathotype (MoT) and Pennisetumpathotype (MsP) to quantify the host status of barley, and to identify sources of blast resistance. Unlike wheat, the barleygene pool is rich with sources of complete and partialresistance againstMagnaporthe. Cultivated barleyappeared a nonhost to MsP, whereas wild barleyshowed some degree of susceptibility. All 153 testedrice accessions were resistant to the MoT isolate,suggesting that rice is nonhost to this pathotype.Inoculation of L94/Vada and Vada/SusPtrit RILmapping populations with MoO and/or MoT isolatesofM. oryzaeindicated one large effect QTL, desig-nated as Rmoq1, on the short arm of chromosome 7Hagainst the MoT isolate PY 67.1 in both populations.Resistance in L94 to the MoO isolate was due to adifferent QTL, located at 5H. An association mappingpanel of West European barley cultivars also sug-gested that most QTLs were pathotype specific. Sixblast resistance genes found in the biparental andassociation mapping studies coincided with mappositions of powdery mildew resistance genes viz.Mlt,Mla6,Mlg,mlo,Mlj, andMlhbgenes. Our QTLand association mapping analyses do not support theassociation of themloresistance gene with enhancedsusceptibility toM. oryzaeas reported in literature. KeywordsAssociation mappingBarleyBlastdiseaseGWASMagnaporthe oryzaemloQTLmapping
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Association mapping, QTL mapping, Mlo, GWAS, Blast fungi, Magnaporthe blast fungi, Magnaporthe oryzae/grisea, Barley, Blast disease, Magnaporthe oryzae, Triticum