Combinación de técnicas in vitro y ex vitro para la micropropagación de Santa Rita (hibr.) Una arbustiva de relevancia ornamental
No hay miniatura disponible
Fecha
Título de la revista
ISSN de la revista
Título del volumen
Editor
Instituto Nacional de Tecnología Agropecuaria
Resumen
Descripción
A protocol combining in vitro and ex vitro techniques was developed topropagate Bougainvillea sp. To sterilize explants, nodal segments were submerged in commercial sodium hypochlorite (5 %) and Tween 80 (0.1 %) for 20 minutes.Subsequently, they were maintained in Plant Preservative Mixture (2 %) underconstant and gentle shaking for 7 hours. Induced and non-induced explants weretested to compare their shoot multiplication rates. The best result was obtainedwhen Murashige-Skoog (MS) basal medium was supplemented with: 5 mg/l 6-benzylaminopurine (BAP), 0.01 mg/l giberellic acid (III), 20 g/l sucrose and a mixtureof antibiotics/antimicotics (1x), pH 5.7, as induction medium. Induced and noninducedexplants were subsequently transferred to MS basal medium supplementedwith constant levels of giberellic acid (III) and decreasing concentration of BAP.Under these culture conditions, induced explants produced 2.5 times more shoots than non-induced ones. Plantlets were rooted cutting the in vitro shoots and inserting them in plugs under hight humidity conditions. Whole plants were recovered 30 to 45 days later and transferred to pots for the acclimatization stage. While rooting percentage was 60 %, rustication percentage was 100 %
Palabras clave
Agrociencias