Isolation of a cotton NADP(H) oxidase homologue induced by drought stress.

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dc.creatorNEPOMUCENO, A. L.
dc.creatorSTEWART, J. M.
dc.creatorOOSTERHUIS, D.
dc.creatorTURLEY, R.
dc.creatorNEUMAIER, N.
dc.creatorFARIAS, J. R. B.
dc.date2011-04-09T17:11:10Z
dc.date2011-04-09T17:11:10Z
dc.date2000-08-30
dc.date2000
dc.date2011-04-10T11:11:11Z
dc.date.accessioned2026-07-07T11:47:28Z
dc.descriptionThe aim of this study was to identify and isolate genes that are differentially expressed in four selected cotton (Gossypium hirsutum L.) genotypes contrasting according to their tolerance to water deficit. The genotypes studied were Siokra L-23, Stoneville 506, CS 50 and T-1521. Physiological, morphological and developmental changes that confer drought tolerance in plants must have a molecular genetic basis. To identify and isolate the genes, the mRNA Differential Display (DD) technique was used. Messenger RNAs differentially expressed during water deficit were identified, isolated, cloned and sequenced. The cloned transcript A12B15-5, a NADP(H) oxidase homologue, was up regulated only during the water deficit stress and only in Siokra L-23, a drought tolerant genotype. Ribonuclease protection assay confirmed that transcription.
dc.identifierPesquisa Agropecuaria Brasileira, Brasilia, v.35, n.7, p.1407-1416, jul. 2000.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/444817
dc.identifier.urihttp://hdl.handle.net/123456789/512752
dc.languageeng
dc.rightsopenAccess
dc.subjectExpressa genica
dc.subjectTransformacao genetica
dc.subjectDeficit hidrico
dc.subjectBiotecnology
dc.subjectWater deficit
dc.subjectRNA mensageiro
dc.subjectAlgodão
dc.subjectGossypium Hirsutum
dc.subjectBiotecnologia
dc.subjectgenetic transformation
dc.subjectgene expression
dc.subjectcotton
dc.subjectmessenger RNA
dc.titleIsolation of a cotton NADP(H) oxidase homologue induced by drought stress.
dc.typeArtigo de periódico

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