Detection of Ralstonia solanacearum (Rs) in latently infected potato stem extracts by post-enrichment qPCR.

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dc.creatorGutarra, L.
dc.creatorFuentes, S.
dc.creatorKreuze, Jan F.
dc.date2012
dc.date2015-05-19T10:56:24Z
dc.date2015-05-19T10:56:24Z
dc.date.accessioned2026-06-27T18:02:53Z
dc.descriptionA sensitive method for detection of Ralstonia solanacearum in latently infected potato stem extracts has been achieved by previous enrichment procedure followed by Real time PCR (qPCR). Sensitivity of qPCR before and after enrichment (48h of incubation of the stem extract with modified SMSA broth at 30oC) was compared with other techniques such as NCM-ELISA and R.solanacearum isolation in Kelman’s medium. Before enrichment procedure, 174.6 cells/ml were detected in Kelman’s medium, 1.71 x 106 cells/ml by NCM-ELISA and 1.29x105 cells/ by qPCR. After enrichment, sensitivities of post enrichment qPCR, NCM- ELISA and isolation on modified Kelman’s medium were similar. As few as 6.56 cells/ml were detected in latently infected potato stem extracts. Serial dilutions of naturally-infected stem extract before enrichment allowed the quantification of populations of R. solanacearum in each stem extract. Post –enrichment qPCR combines the advantages of high sensitivity, ease and speed, but it requires expensive laboratory equipment. Thus it can be used by seed and breeding programmes in developing countries only to confirm positive results obtained by serological tests used for seed quality control and for assessing susceptibility of breeding lines to bacterial wilt.
dc.formatapplication/pdf
dc.identifierhttps://hdl.handle.net/10568/66293
dc.identifier.urihttp://hdl.handle.net/123456789/153426
dc.languageen
dc.rightsOpen Access
dc.sourceGutarra, L.; Segundo, F.; Kreuze, J. 2012. Detection of Ralstonia solanacearum (Rs) in latently infected potato stem extracts by post-enrichment qPCR. Memorias de la 58ava reunión anual: Programas y resumenes. 58. Annual Meeting of The Interamerican Society for Tropical Horticulture (ISTH). 16. Congreso de la Sociedad Peruana de Horticultura. Lima (Perú). 3 - 6 Sep 2012. Lima (Peru). ISTH Universidad Nacional Agraria La Molina (UNALM). p. 64. Abstract
dc.subjectpotatoes
dc.subjectralstonia solanacearum
dc.subjectbacterial diseases
dc.subjectplant extracts
dc.subjectplant diseases
dc.subjectlatent infections
dc.subjectpcr
dc.subjectmethods
dc.titleDetection of Ralstonia solanacearum (Rs) in latently infected potato stem extracts by post-enrichment qPCR.
dc.typeConference Paper

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