Molecular characterisation of isolates of Metarhizium from locusts and grasshopers

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Springer

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The relationships between 30 strains of Metarhizium were investigated by isoenzyme analysis, PCRRAPDs, and protease production. The strains included representatives of M. anisopliae, M. Flavoviride var. ¯avoviride and M. Flavoviride var. minus. Thirteen isolates conforming to M. Flavoviride from acridoid hosts and pathogenic to locusts and grasshoppers were shown to be a single, distinctive genotype with a pan-tropical distribution. In addition, the separation of M. Flavoviride var. minus as a host specific taxon distinct from European isolates of M. Flavoviride was also supported. The possibility of coevolution within some insect pathogenic populations is discussed.

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metarhizium anisopliae, biological control, rapd, isoenzymes, locusts, grasshoppers

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