Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Alfalfa mosaic virus (AMV) in legume crops applied by ICARDA’s Seed Health and Virology Laboratory

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dc.creatorKumari, Safaa
dc.creatorMoukahel, Abdulrahman
dc.date2024-11-12
dc.date2024-11-14T15:21:34Z
dc.date2024-11-14T15:21:34Z
dc.date.accessioned2026-06-27T14:22:23Z
dc.descriptionAlfalfa mosaic virus (AMV, genus Alfamovirus, family Bromoviridae) is a significant plant pathogen affecting a wide range of legume crops across various regions of the world including West Asia and North Africa (WANA) countries. AMV is transmitted by aphids in a non-persistent manner and via seeds of several hosts (including faba bean, chickpea, and lentil). Seed transmission makes managing AMV more challenging, as infected seeds can introduce the virus into new areas and lead to infections in the next growing season, even before aphid vectors are active. Accordingly, seed testing and the use of certified seeds are key strategies to mitigate the spread of AMV as a seed-borne virus. Early detection and accurate diagnosis of viral diseases is critical for the application of appropriate control measures. However, the accuracy and reliability of molecular methods is a fact beyond dispute. This report describes a validated molecular tool for AMV detection and characterization applied by ICARDA’s Seed Health and Virology Laboratory.
dc.formatapplication/pdf
dc.identifierhttps://hdl.handle.net/10568/159753
dc.identifier.urihttp://hdl.handle.net/123456789/77734
dc.languageen
dc.publisherInternational Center for Agricultural Research in the Dry Areas
dc.rightsOpen Access
dc.sourceSafaa G. Kumari and Abdelrahman Moukahel. 2024. Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Alfalfa mosaic virus (AMV) in legume crops applied by ICARDA’s Seed Health and Virology Laboratory. International Center for Agricultural Research in the Dry Areas (ICARDA), Beirut, Lebanon.
dc.subjectfaba bean
dc.subjectlentil
dc.subjectchickpea
dc.subjectalfalfa
dc.subjectlegume
dc.subjectcommon bean
dc.subjectgrass pea (lathyrus sativus)
dc.subjectmedics
dc.subjectlegume viruses
dc.subjectrt-pcr
dc.subjectseedborne diseases
dc.subjectic-rt-pcr
dc.subjectlegume
dc.subjectfield pea (pisum sativum)
dc.titleReverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Alfalfa mosaic virus (AMV) in legume crops applied by ICARDA’s Seed Health and Virology Laboratory
dc.typeTool

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