CLONAL PROPAGATION OF NEEM (Azadirachta indica A. Juss.) VIA DIRECT AND INDIRECT IN VITRO REGENERATION

Laureen Michelle   Houllou; Robson Antônio   de Souza; Elizabete Cristina   Pacheco dos Santos; José Jackson   Pereira da Silva; Marta   Ribeiro Barbosa; Jean Philippe   Guimarães Sauvé; Wolfgang   Harand

CLONAL PROPAGATION OF NEEM (Azadirachta indica A. Juss.) VIA DIRECT AND INDIRECT IN VITRO REGENERATION

Autores

Laureen Michelle Houllou

Robson Antônio de Souza

Elizabete Cristina Pacheco dos Santos

José Jackson Pereira da Silva

Marta Ribeiro Barbosa

Jean Philippe Guimarães Sauvé

Wolfgang Harand

Editor

Universidade Federal de Viçosa

Descripción

ABSTRACT – The study was conducted with shoot tip explants of neem (Azadirachta indica A. Juss)to identify a viable regenerative process. Shoot tips were obtained from neem embryos cultured alternatinglyin DKW medium supplemented with BAP and medium without hormones. Initial shoot developmentwas influenced by cotyledon presence. Basal callus, excised from in vitro stem base, also presented organogenicpotential. In some cases, plant lines, obtained from each seed, presented different characteristics. Themost common characteristic observed in vitro was callus formation at the stem base. However, therarest characteristics were stem callus formation and leaf senescence. The regenerated shoot tips werefurther subculture and rooted on a medium supplemented with IBA so that complete plants could beobtained. The rooted plants were transplanted to a greenhouse and successfully acclimatized. No significantdifferences in in vivo development were observed between neem plants from callus and from shoot tippropagation.