Mini-Percoll processing of domestic ruminant frozen-thawed semen dispenses the use of heparin in capacitating medium.

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dc.contributorCarolina Cerqueira Sarmento Olivares, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brasil
dc.contributorJoanna Maria Gonçalves Souza-Fabjan, UFF - Niterói, RJ, Brasil
dc.contributorUniversidade do Grande Rio (Unigranrio) - Duque de Caxias, RJ, Brasil.
dc.contributorJEFERSON FERREIRA DA FONSECA, CNPC
dc.contributorRibrio Ivan Tavares Pereira Batista, UFF - Niterói, RJ, Brasil
dc.contributorMario Felipe Alvarez Balaro, UFF - Niterói, RJ, Brasil
dc.contributorHelena Fabiana Reis de Almeida Saraiva, UFF - Niterói, RJ, Brasil
dc.contributorVivian Angélico Pereira Alfradique
dc.contributorLuana Rangel Côrtes, UFF - Niterói, RJ, Brasil
dc.contributorFelipe Zandonadi Brandão.
dc.creatorOLIVEIRA, C. C. S.
dc.creatorSOUZA-FABJAN, J. M. G.
dc.creatorFONSECA, J. F. da
dc.creatorBATISTA, R. I. T. P.
dc.creatorBALARO, M. F. A.
dc.creatorSARAIVA, H. F. R. de A.
dc.creatorALFRADIQUE, V. A. P.
dc.creatorCÔRTES, L. R.
dc.creatorBRANDÃO, F. Z.
dc.date2017-11-27T23:35:35Z
dc.date2017-11-27T23:35:35Z
dc.date2017-11-27
dc.date2017
dc.date2019-09-23T11:11:11Z
dc.date.accessioned2026-07-01T00:20:50Z
dc.descriptionSperm capacitation is a prerequisite for mammal successful fertilization. Although usually a capacitating substance such as heparin is used during sheep in vitro fertilization, evidences suggest that the cryopreservation process and Percoll technique could induce spontaneous capacitation. This study aimed to compare ovine, caprine and bovine frozen-thawed sperm after mini-Percoll processing on sperm parameters, receiving or not heparin supplementation. In conclusion, frozen-thawed ovine, caprine and bovine spermatozoa processed with mini-Percoll behave similarly regarding to capacitation status and does not require heparin supplementation during in vitro incubation to achieve capacitation. [Processamento pela técnica de mini-Percoll em sêmen congelado/descongelado de ruminantes domésticos dispensa o uso da heparina em meio capacitante].
dc.descriptionEdição dos anais do XXII Congresso Brasileiro de Reprodução Animal (CBRA), Santos, SP, Brasil, maio 2017.
dc.identifierRevista Brasileira de Reprodução Animal, Belo Horizonte, v. 41, n. 1, p. 498, jan./mar. 2017.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1080820
dc.identifier.urihttp://hdl.handle.net/123456789/402282
dc.languageeng
dc.rightsopenAccess
dc.subjectCapacitação espermática
dc.subjectOvino
dc.subjectCaprino
dc.subjectBovino
dc.subjectReprodução animal
dc.subjectSêmen
dc.subjectHeparina
dc.subjectSheep
dc.subjectGoats
dc.subjectCattle
dc.subjectSpermatozoa
dc.subjectReproduction
dc.subjectIn vitro fertilization
dc.subjectHeparin
dc.subjectsperm capacitation
dc.titleMini-Percoll processing of domestic ruminant frozen-thawed semen dispenses the use of heparin in capacitating medium.
dc.typeResumo em anais e proceedings

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