Stomata counting automation facilitates C. canephora tetraploid selection.
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Chromosome doubling can enhance reproductive compatibility between genotypes with different ploidy levels. We ́ve been using anti-mitotics to double a C. canephora clone chromosome number in order to facilitate nematode resistance introgression to C. arabica cultivars. Tetraploid plants will be homozygous, and will be potentially able to contribute complete resistance gene sets for interspecific hybrid progenies. Once treated with anti-mitotics, the plants had the stomata counted as the initial screening for tetraploids. The chromosome number is inversely correlated to the stomata number in diploid and tetraploid C. canephora plants [1]. Stomata counting is, nevertheless, time consuming and monotonous. The objective of this work was to test a software tool to identify and quantify stomata and compare scores with human scores produced by different people.
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Software, Anti-mitotic, Coffea Canephora, Coffea Arábica