Electroferotipificación de cepas de rotavirus de origen porcino colombianas

dc.audienceInvestigador
dc.audienceTécnico
dc.audienceProfesional
dc.audienceProductor
dc.coverageColombia
dc.creatorValencia F., Carolina
dc.creatorAriza Botero, Manuel Fernando
dc.creatorGutiérrez, María Fernanda
dc.creatorBarrera Velandia, José del Carmen
dc.creatorMogollón García, Manuel Esteban
dc.date2022-10-31T13:45:53Z
dc.date2022-10-31T13:45:53Z
dc.date1996-01
dc.date1996
dc.date.accessioned2026-06-27T04:37:27Z
dc.descriptionMediante la técnica de electroforesis (PAGE) se analizaron 204 muestras fecales de lechones diarreicos entre dos y seis semanas de edad, de diferentes granjas porcícolas del país, con el fin de analizar los electroferotipos del ARN de rotavirus. Para la extracción del ARN viral se empleó tiocianato de guanidina y la electroforesis se realizó en geles de poliacrilamida. Sólo 33 muestras ( 16.1 %) fueron positivas al ARN de rotavirus.
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dc.identifier0122-0985
dc.identifierhttp://hdl.handle.net/20.500.12324/37507
dc.identifierreponame:Biblioteca Digital Agropecuaria de Colombia
dc.identifierrepourl:https://repository.agrosavia.co
dc.identifierinstname:Corporación colombiana de investigación agropecuaria AGROSAVIA
dc.identifier.urihttp://hdl.handle.net/123456789/33398
dc.languagespa
dc.publisherCorporación Colombiana de Investigación Agropecuaria - CORPOICA
dc.relationRevista del CEISA
dc.relation3
dc.relation1
dc.relation50
dc.relation64
dc.relationBohl, E.H.; Kholer, E.M.; Saif, l. J.; Cross, R.F.; Agnes, A.G.; Theil, -, K.W. 1978. Rotavirus as a cause of diarrhea in pigs. J. Am. Vet. Med. Ass. 172: 458-462.
dc.relationCaetano, C.A.; Gresshoff, P.M. 1993. Staining nucleic acids with silver and alternative to radioisotipic and fluorescent labeling. Promega Notes. 45: 13-18.
dc.relationCiarlet, M.; Liprandi, F. 1994. Serological and genomic chai:acterization of two porcine rotaviruses with serotype G1 ·specificity. J. Clin. Microbio!. 32: 269-272.
dc.relationCosta, V.M.; Beer, M.; Peenze, Y.; Steele, A.D. 1993. Molecular . epidemiology and ~ubgroup analysis of bovine group A rotaviruses associated with diarrhea in South African calves. J. Clin. Microbio!. Jl: 3333-3334.
dc.relationChomezynski, P.; Sacchi, N. 1987. Single-step method of ARN isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156-159.
dc.relationDyall-~_~ith, M.l.; Holmes, I.H. 1981. Gene-coding assignements of rotavirus double standed RNA segments 1 O and 11. J. Vi rol. 38: 1099- 1103.
dc.relationEstes, M.K.; Graham, D.Y.; Dimitrov, D.M. 1984. The molecular ,., epidemiology of rotavirus gastroenteritis. Prog. Med. Viro!. 29: 1-22.
dc.relationFang, Z.Y.; Glass, R.I.; Peñaranda, M.; Dang, H.; Monroe, S.S.; Wen, l.; Eiden, J.; Yolken, R.H.; Saif, L.; Gouvea, V.; Hung, T. 1989. Purification and characterization of adult diarrhea rotavirus; ldentification of viral structural proteins. J. Viro!. 63: 2191-2197.
dc.relationFitjman, N. L.; Barrandeguy, M. E.; Cornaglia, Em.M.; Schudel, A.A. 1987. Variations and persistency of electropherotypes of bovine rotavirus field isolates. Ach. Viro!. 96: 275-281.
dc.relationGonzález, G.; Torres, M.; Mogollón, J.D.; Gómez, A. 1992. Características del •·ritavirus porcino aislado en Colombia. Informe Veterinario CE1-7.
dc.relationGouvea, V.; Santos, N.; Carmo Timenetsky, M. 1994. VP4 typing of bovine and porcine group A rotaviruses by PCR. J. Clin. Microbio!. 32: 1333-1337.
dc.relationGreen, K.Y.; Hoshino, Y.; lkegami, N. 1989. Sequence analysis of the gene encoding the ser_otype-specific glyucoprotein NP7) of two new human rotavirus serotypes. Virology. 168: 429-433.
dc.relationHasega\va, A.; lnoye, S.; Matsuno, S.; Yamoaka, K.; Eko, R.; Suharyono, W. 1984,, lsolation of human rotaviruses with a distinct RNA electrophoretic pattern from Indonesia. Microbio! lmnJ.!!!lol. 28: 719-722.
dc.relationHerring, A.J.; lnglis, M.F.; Ojeh, C.K.; Snodgrass, D.R.;_~enzies, J.D. 1982. Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J. Clin. Microbio!. 1§: 473-477.
dc.relationJanke, B.H.; Nelson, J.K.; Benfield, D.A.; Nelson, E.A. 1990. Relative prevalence of typical and atypical strains among rotaviruses from diarrheic pigs in conventional swine herds. J. Vet. Diagn. lnvest. .2_: 308-311.
dc.relationKaminjolo, J. S.; Adesiyun, A.A. 1994. Rotavirus infection in calves, piglets, lambs and goat kids in Trinidad. Br. Vet. J. 150: 293-299.
dc.relationLeammli, U.K. 1970. Cleave of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227: 680-685.
dc.relationLi, B.; Gorziglia, M. 1993. VP4 serotype of the Gottfried strains of porcine rotavirus. J. Clin. Microbio!. J]_: 3075-3077.
dc.relationLópez, S.; Padilla, L.; Arias, S.F.Z. 1993. Correlación entre serotipo y electroferotipo de rotavirus aislados en dos poblaciones humanas de México. Bol. Med. Hosp. lnfant. Mex. 50: 736-740.
dc.relationMase~deyez, P.J.; Unicomb, L.E.; Kirkwood, C.D.; Bishop, 'R.F. 1994. Rotavir_us serotypes caysing severe acute diarrhea in joung children in six Australian cities, 1989 to 1992. J. Clin. Microbio!. 32: '2315-2317.
dc.relationNa?esh~, H.S.; Brown, LE.; Holmes, I.H. 1989. Neutralizing monoclonal antibod1es against three serotypes of porcine rotavirus. J. Vi rol. 63: 3545- 3549.
dc.relationParwani, W.V.; Hussein, H.A.; Rosen, B.I.; Luchelli, A.; Navarro, L.; Saif, L.J. 1993. Characterization of field strains of group A bovine rotaviruses by using polymerase chain reaction-generated G and P type-specific cDN_A probes. J. Clin. Microbio!. _11: 2010-2015.
dc.relationPedley, S.; Bridger, J.C.; Mccrae, M.A. 1983. Molecular analysis of rotavirus isolates that lack the group antigens. Science. 23: 157-163.
dc.relationPocock, D.H. 1987. Characterization of rotavirus isolates frorn subclinically infected calves by genome profile analysis. -Vét. Microbio!. U: 27-34.
dc.relationPuerto, M.; Puerto, F.I.; Polanco, G.G.; Goní:ález, M.R.; Alvarez, M.; Rodríguez, J.C. 1994. Detección de rotavirus en lechones lactantes de granjas localizadas en el estado de Yucatán. Vet. Mex. 25: 69-71.
dc.relationRosen, B.I.; Parwani, AJ/.; López, S.; Flórez, l.; Saif, L.J. 1994. Serotypic differentiation of rotaviruses in field samples from diarrheic pigs by using nucleic acid probes specific far porcine VP7 and human and porcine VP7 genes. J. Clin. Microbio!. 32: 311-317.
dc.relationTheil, K.; Saif, L.J.; Moorhead, P.D.; Whitmoyer, R.W. 1985. Porcine rotavirus like virus (group B rotavirus): Characterization and pathogenicity far gnotobiotic pigs. J. Clin. Microbio!. l]_: 340-345.
dc.relationTodd, D.; Mcnully, M.S.; Allan, G.M. 1984. The use of polyacrylamide gel electrophoresis of virus RNA in the study of rotavirus infections. Recent advances in virus diagnosis. ~: 125-135.
dc.relationTsunemitsu, H.; Saif, L.J.; Jiang, D.; Shimizu, M.; Hiro, M.; Yanaguchi, H.; lshiyama, T.; Hirai, T. 1991. lsolation, characterization and serial propagation of a bovine group C rotavirus in a monkey kidney cell line (MA-104). J: Clin. Microbio!. 29: 2609-2613.
dc.relationUtrera, V.; Mazzali De llja, R.; Gorziglia, M.; Esparza, J. 1984. Epidemiological aspects of porcine rotavirus infection in Venezuela. Res. Vet. Sci. 36: 310-315.
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourceRevista del CEISA; Vol. 3, Núm. 1. (1996): Revista del CEISA (Enero-Junio);p.50-64.
dc.subjectEnfermedades de los animales - L73
dc.subjectCerdos cebón
dc.subjectRotavirus porcino
dc.subjectElectrofisiología
dc.subjectEnfermedades de los animales
dc.subjectGanadería y especies menores
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_59e0f842
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_34753
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_6755a45d
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_426
dc.thumbnailhttps://repository.agrosavia.co/bitstreams/213560cf-52d2-4f1e-8ebb-84affcf2eef0/download
dc.titleElectroferotipificación de cepas de rotavirus de origen porcino colombianas
dc.typeArtículo científico

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