DNA POLYMORPHISMS IN CUBAN VARIETIES OF AVOCADO (Persea americana Mill.) AS DETECTED BY INVERSE SEQUENCE TAGGED REPEAT (ISTR) ANALYSIS
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Instituto Nacional de Ciencias Agrícolas
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A survey of the genetic diversity among Cuban commercial avocado varieties was initiated using ISTR analysis. Dice¿s dissimilarities between genotype pairs were calculated. A cluster analysis was performed based on dissimilarity using UPGMA as the clustering method. ISTR markers were efficient in detecting polymorphisms among the genotypes. The dissimilarity values ranged from 0.24 between var. "Suardía" and "Hass" to 1.00 between "Lula" and "Los Moros" or "CHI- 3" with an average dissimilarity of 0.78. The efficiency of UPGMA in estimating genetic relationships between varieties was corroborated by the cophenetic correlation coefficient, which indicated that the distortion degree in the estimated dissimilarities was minimal. Ecological groups were not adequately represented in the dendrogram. Thus, West Indians, Guatemalan and Mexican genotypes were positioned across the dendrogram. The utility of ISTR for genotype identification and assessment of genetic diversity in commercial avocado varieties is discussed.
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Agrociencias