Carbon-dot pequi-nut in the development of immunosensor to detect pathogenic bacteria.

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dc.contributorRACHEL MENEZES CASTELO, UNIVERSIDADE ESTADUAL DO CEARÁ
dc.contributorMARÍLIA DE ALBUQUERQUE OLIVEIRA, RENORBIO
dc.contributorROSELAYNE FERRO FURTADO, CNPAT
dc.contributorBRUNO PEIXOTO DE OLIVEIRA, UNIVERSIDADE FEDERAL DO CARIRI
dc.contributorLUCAS VINICIUS LEITE MARTONI, UNIVERSIDADE DE SÃO PAULO
dc.contributorTEREZINHA FEITOSA MACHADO, CNPAT
dc.contributorCELLI RODRIGUES MUNIZ, CNPAT
dc.contributorFLÁVIA OLIVEIRA MONTEIRO DA SILVA ABREU, UNIVERSIDADE ESTADUAL DO CEARÁ
dc.contributorSÉRGIO ANTÔNIO SPINOLA MACHADO, UNIVERSIDADE DE SÃO PAULO
dc.contributorAIRIS MARIA ARAÚJO MELO, INSTITUTO FEDERAL DO PIAUÍ CAMPUS PICOS
dc.contributorHUAI N. CHENG, SOUTHERN REGIONAL RESEARCH CENTER
dc.contributorATANU BISWAS, USDA AGRICULTURAL RESEARCH SERVICE
dc.contributorCARLUCIO ROBERTO ALVES, UNIVERSIDADE ESTADUAL DO CEARÁ.
dc.creatorCASTELO, R. M.
dc.creatorOLIVEIRA, M. DE A.
dc.creatorFURTADO, R. F.
dc.creatorOLIVEIRA, B. P. DE
dc.creatorMARTONI, L. V. L.
dc.creatorMACHADO, T. F.
dc.creatorMUNIZ, C. R.
dc.creatorABREU, F. O. M. DA S.
dc.creatorMACHADO, S. A. S.
dc.creatorMELO, A. M. A.
dc.creatorCHENG, H. N.
dc.creatorBISWAS, A.
dc.creatorALVES, C. R.
dc.date2025-06-11T19:47:52Z
dc.date2025-06-11T19:47:52Z
dc.date2025-06-11
dc.date2025
dc.date.accessioned2026-06-30T23:05:45Z
dc.descriptionCarbon dots in biosensing have advanced significantly, adding improvements to different detection techniques. In this study, an amperometric immunosensor for Salmonella Thyphimurium was designed using antibodies labeled with car- bon dots (Cdots) from pequi almond (Caryocar brasiliensis). Cdots were synthesized by pyrolysis and characterized by FTIR, UV/fluorescence, electrochemistry, zeta potential, and transmission electron microscopy (TEM). A particle size of 6.80 ± 2.13 nm was estimated, and the zeta potential was − 47.4 mV, indicating the preponderant presence of acidic groups, as confirmed by FTIR. The impedance evaluation of the response of biosensors assembled for live (Rct = 13.4 kΩ) and dead (Rct = 499.7 Ω) Salmonella showed a significant difference in their values, in agreement with chronoamperometric analyses, which had their current values drastically reduced from − 2.2 mA (live) to 0 mA (dead). An analytical curve for Salmonella was established with the limit of detection lower than 1 CFU/mL. This electrochemical biosensor using pequi carbon dots for antibody labeling showed promising results for detecting the pathogen. Thus, carbon dots can be used as substitutes for enzymes in labeling antibodies used in the design and production of sensors.
dc.identifierBrazilian Journal of Microbiology, Jan. 2025
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1176555
dc.identifierhttps://doi.org/10.1007/s42770-025-01612-1
dc.identifier.urihttp://hdl.handle.net/123456789/374017
dc.languageeng
dc.rightsopenAccess
dc.subjectCarbon dots
dc.subjectCaryocar brasiliensis Cambess
dc.subjectPequi nut
dc.subjectSalmonella Thyphimurium
dc.subjectPontos de carbono
dc.subjectImunosensor
dc.subjectNoz de pequi
dc.subjectImmunosensors
dc.titleCarbon-dot pequi-nut in the development of immunosensor to detect pathogenic bacteria.
dc.typeArtigo de periódico

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