Characterisation of the QM gene of Trypanosoma brucei

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dc.creatorLillico, Simon G.
dc.creatorMottram, J.C.
dc.creatorMurphy, N.B.
dc.creatorWelburn, S.C.
dc.date2002-06
dc.date2013-07-03T05:25:45Z
dc.date2013-07-03T05:25:45Z
dc.date.accessioned2026-06-27T17:09:12Z
dc.descriptionThe QM protein has been reported to have roles in both tumour suppression and transcription factor regulation in vertebrate cells, and in ribosome stability in both yeast and mammals. The present study isolated the QM gene of Trypanosoma brucei and determined its sequence. Alignment with QM sequences from Saccharomyces cerevisiae, Arabidopsis thaliana, Drosophila melanogaster and Homo sapiens revealed greater than 60% identity. Southern blot analysis revealed multiple copies of QM within the trypanosome genome. An epitope tag was inserted into the C-terminus of the T. brucei QM and the protein expressed under inducible control in procyclic form trypanosomes. Immune fluorescence microscopy revealed co-localisation with the GPI:protein transamidase component, GPI8, a distribution indicative of ribosome association with the rough endoplasmic reticulum.
dc.identifierhttps://hdl.handle.net/10568/32920
dc.identifier.urihttp://hdl.handle.net/123456789/143777
dc.languageen
dc.publisherOxford University Press
dc.rightsOpen Access
dc.sourceFEMS Microbiology Letters;211(2): 123-128
dc.subjecttrypanosoma brucei
dc.subjectgenes
dc.subjectproteins
dc.subjectendoplasmic reticulum
dc.subjectnucleotide sequence
dc.titleCharacterisation of the QM gene of Trypanosoma brucei
dc.typeJournal Article

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