Phylogenetic analysis and molecular characterization of BBTV DNA-R of wild and cultivated banana isolates from East Java, Indonesia

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dc.creatorDidik Wahyudi
dc.creatorIhda Ummu Aufa
dc.creatorLia Hapsari
dc.date2023
dc.date.accessioned2026-07-07T03:29:40Z
dc.descriptionBanana bunchy top virus (BBTV) molecular detection and understanding its origin are important issues for mitigating future spread. The aim of this study was to molecularly detect BBTV infection and analyze the characteristic also phylogenetic of banana isolates from East Java Indonesia. Two BBTV asymptomatic wild bananas and two BBTV symptomatic banana cultivars were examined. PCR amplifications were accomplished using BBTV DNA-R primers for master replication initiation protein. Sequences evaluations were conducted in SeqScanner. Sequences identification was performed in nucleotide BLAST. Translation of ORFs was determined using ORF Finder server tool. Protein identification was conducted in protein BLAST. Sequences polymorphisms were analyzed using DnaSP6. Phylogenetic analysis was employed using Neighbor-Joining algorithm with Kimura two-parameter (K2P) substitution model in MEGA7. Results showed that BBTV DNA-R components were detected in all isolates and confirmed as Rep protein. The sequences length were varied from 616 to 1,074 bp, low GC content (42.90%) and low conservation (56.47%). Asymptomatic wild bananas generated shorter length and more variable sequences, presumably related to the resistance mechanism. Phylogenetic analysis of BBTV DNA-R East Java with other 38 homolog sequences worldwide were found clustered in Asian Group, closely related to Vietnam, Thailand, and China. Hence, it presumably originated from China mainland via Malay Peninsula route.
dc.formatapplication/pdf
dc.identifier0304-2847
dc.identifierhttps://www.redalyc.org/articulo.oa?id=179976349003
dc.identifierhttps://www.redalyc.org/journal/1799/179976349003/
dc.identifierhttps://www.redalyc.org/journal/1799/179976349003/html/
dc.identifierhttps://www.redalyc.org/journal/1799/179976349003/179976349003.epub
dc.identifierhttps://www.redalyc.org/journal/1799/179976349003/movil
dc.identifier10.15446/rfnam.v76n3.104708
dc.identifier.urihttp://hdl.handle.net/123456789/435298
dc.languageen
dc.publisherUniversidad Nacional de Colombia
dc.relationhttp://www.redalyc.org/revista.oa?id=1799
dc.rightsRevista Facultad Nacional de Agronomía - Medellín
dc.sourceRevista Facultad Nacional de Agronomía - Medellín (Colombia) Num.3 Vol.76
dc.subjectAgrociencias
dc.titlePhylogenetic analysis and molecular characterization of BBTV DNA-R of wild and cultivated banana isolates from East Java, Indonesia
dc.typeartículo científico

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Revista Facultad Nacional de Agronomía - Universidad Nacional de Colombia