A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells

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dc.creatorBallingall, Keith T.
dc.creatorMwangi, David Miano
dc.creatorMacHugh, Niall D.
dc.creatorTaracha, E.L.N.
dc.creatorTotte, P.
dc.creatorMcKeever, Declan J.
dc.date2000-05
dc.date2013-07-03T05:25:55Z
dc.date2013-07-03T05:25:55Z
dc.date.accessioned2026-06-27T17:19:51Z
dc.descriptionWe describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effectiveness of this assay in detecting rBoIFN y and activation of immune CD4 - and CD8 - T cell lines and clones in response to specific antigen and transfected COS-7 cells, respectively. We also demonstrate its utility in identifying purified pathogen fractions that activate immune CD4-T cell clones.
dc.identifierhttps://hdl.handle.net/10568/33010
dc.identifier.urihttp://hdl.handle.net/123456789/149074
dc.languageen
dc.publisherElsevier
dc.rightsLimited Access
dc.sourceJournal of Immunological Methods;239: 85-93
dc.subjectcattle
dc.subjectantigens
dc.subjectbovidae
dc.subjectcells
dc.subjectantibodies
dc.subjecttheileria parva
dc.titleA highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells
dc.typeJournal Article

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Instituto Internacional de Investigaciones Pecuarias (ILRI)